猪繁殖与呼吸综合症流行株ORF_5基因遗传变异分析  

Analysis on Heredity and Variation of the ORF5 Gene of Prevalence Strains Porcine Reproductive and Respiratory Syndrome Virus

作　　者：尹国友[1] 孙婕[1] 陈兰英[1] 赵祯[1] 

机构地区：[1]河南城建学院生物工程系,河南平顶山467044

出　　处：《安徽农业科学》2010年第8期4115-4117,共3页Journal of Anhui Agricultural Sciences

摘　　要：[目的]分析猪繁殖与呼吸综合症发病原因,流行趋势,为预防和控制猪繁殖与呼吸综合症病毒(PRRSV)提供理论依据。[方法]根据GenBank上的PRRSV的ATCCVR-2332的全基因序列,设计并合成引物,采用RT-PCR技术扩增了7株流行株中的ORF5基因序列。应用DNAStar软件分析所测序列,并与ATCCVR-2332、CH-1a、BJ-4、LV及疫苗株MLV进行序列比较。同时用系统进化树分析了分离株之间的系统进化树。[结果]核苷酸序列分析表明这7株流行株中ORF5基因与VR-2332、CH-1a、BJ-4等代表株的同源性分别为88.1%～98.8%,89.9%～95.2%,85.6%～98.7%;与LV的同源性为54.7%～56.9%。氨基酸序列分析表明,这7株流行株与VR-2332、CH-1a、BJ-4等代表株的同源性分别为88.1%～96.8%,88.1%～94.5%,86.1%～96.5%;与LV的同源性为54.7%～56.2%。[结论]流行株在ORF5基因区域的变异较大,同一地域分离株间ORF5基因序列同源性较高且亲缘关系较近,不同地域则较远。[Objective] The aim was to analyze the reason and epidemic trend of PRRSV,and provide theoretical basis for preventing and controlling PRRSV.[Method] According to the sequence of ATCC VR-2332 strain porcine reproductive and respiratory syndrome virus published by the GenBank,the primers were designed and synthesized.ORF5 gene sequences of seven prevalence strains were amplified by RT-PCR.The sequences of ORF5 genes were analyzed by DNAStar and compared with those of ATCC VR-2332,CH-1a,BJ-4,LV-M96262 and MLV vaccine strains,phylogenetic tree among isolates was analyzed.[Result] Analysis of nucleotide sequence showed that the homology was 88.1%-98.8%,89.9%-95.2%,85.6%-98.7% between ORF5 genes of seven prevalence strains and VR-2332,CH-1a,BJ-4,and the homology was 54.7%-56.9% between ORF5 genes and LV.Analysis of amino acid sequence showed that the homology was 88.1%-96.8%,88.1%-94.5%,86.1%-96.5% between ORF5 genes of seven prevalence strains and VR-2332,CH-1a,bBJ-4,the homology was 54.7%～56.2% between the ORF5 genes and LV.[Conclusion] The variation of prevalence strains was great in the ORF5 gene region,the homology of ORF5 gene sequence was higher and genetic relationship was near during prevalence strains in the same region,or was far in different regions.

关 键 词：猪繁殖与呼吸综合症病毒 ORF5基因 序列分析 

分 类 号：S858.28[农业科学—临床兽医学]