血浆肿瘤坏死因子受体相关因子-4升高对炎症性肠病的临床意义  

Role of Elevated Plasma Tumor Necrosis Factor Receptor -Associated Factor- 4 in Patients with Inflammatory Bowel Disease

作　　者：沈骏[1] 冉志华[1] 童锦禄[1] 蔡青[1] 黄美兰[1] 王天蓉[1] 钱孝先[1] 张凤[1] 

机构地区：[1]上海交通大学医学院附属仁济医院消化内科上海市消化疾病研究所,上海200001

出　　处：《医学新知》2010年第1期12-15,共4页New Medicine

基　　金：国家自然科学基金项目（30770964）

摘　　要：目的分析血浆肿瘤坏死因子受体相关因子-4（tumor necrosis factor receptor—associated factor-4，TRAF4）水平对于炎症性肠病的诊断价值以及与内镜下疾病活动性的相关性。方法应用酶联免疫吸附试验（enzyme—linked immunosorbent assay，ELISA）分析炎症性肠病患者和正常对照者血浆中TRAF4蛋白的表达，受试者工作特征曲线（receiver—operating characteristic，ROC）分析血浆TRAF4水平对于克罗恩病和溃疡性结肠炎的诊断价值，应用Pearson相关分析研究血浆TRAF4水平与内镜下疾病活动性的相关性。数据处理使用GraphPad Prism 5。结果克罗恩病患者和溃疡性结肠炎患者血浆TRAF4水平均显著高于正常对照者（P〈0．05）；克罗恩病患者和溃疡性结肠炎患者血浆TRAF4表达水平和内镜下疾病活动指数均无显著的相关性（P〉0．05）。结论炎症性肠病患者血浆TRAF4水平增高，对区分炎症性肠病患者和正常对照者具有诊断价值，但血浆中TRAF4的水平不能反映内镜下疾病活动程度。Objective To analyze the diagnostic value of plasma tumor necrosis factor receptor - associated factor- 4 （TRAF4） in inflammatory bowel disease and its correlation with endoscopic disease activity. Methods Enzyme- Linked ImmunoSorbent Assay （ELISA） was applied to evaluate plasma TRAF4 levels in patients with inflammatory bowel disease and healthy controls. Receiver - operating characteristic （ROC） curves were used to determine the diagnostic value of plasma TRAF4 in Crohn＇s disease and ulcerative colitis. Pearson correlation coefficient was used to analyze the correlation between plasma TRAF4 and endoscopic disease activity. Data were analyzed using GraphPad Prism 5. Results Plasma levels of TRAF4 were significantly higher in Crohn ~ disease （ P = 0.000） and ulcerative colitis （P =0. 000） than in healthy controls. Moreover, TRAF4 showed significant diagnostic value in distin- guishing patients with Crohn＇s disease （P =0.000） and ulcerative colitis （P =0. 000） from healthy controis. However, no significant correlation was indicated between plasma levels of TRAF4 and endoscopic disease activity in patients with Crohn~ disease（ r =0. 150, P = 0.244）or ulcerative colitis （r =0. 168, P =0. 184）. Conclusion Plasma levels of TRAF4 increased in patients with inflammatory bowel disease, which showed diagnostic value in distinguishing patients with inflammatory bowel disease and healthy controls. However, plasma levels of TRAF4 could not reflect endoscopic disease activity.

关 键 词：肿瘤坏死因子受体相关因子-4 克罗恩病 溃疡性结肠炎 内镜下疾病活动程度 

分 类 号：R574[医药卫生—消化系统]