耐酸性硫酸盐还原功能菌株WF83的分离鉴定及生长特性研究  

Identification and Study of Growth Factor of a Sulfate-Reducing Bacterium Strain WF83

作　　者：王方[1] 方迪[1] 单红仙[1] 赵阳国[1] 张瑞昌[1] 

机构地区：[1]中国海洋大学环境科学与工程学院,山东青岛266100

出　　处：《河南科学》2010年第4期415-418,共4页Henan Science

基　　金：国际科学基金项目(IFS;F/4737-1);山东省自然科学基金项目(ZR2009BQ022)

摘　　要：运用Hungate厌氧技术,从某酸性底泥中分离到一株耐酸且具有硫酸盐还原功能的菌株WF83,采用形态以及16SrRNA基因序列分析手段对其鉴定,并对WF83的主要生长因素(pH和温度)进行研究.结果表明,菌株WF83为革兰氏阴性菌,杆状,具有硫酸盐还原功能,产H2S,兼性厌氧;与Enterobacter sp.的同源相似水平达99%,初步鉴定该菌株可能为Enterobacter属.生长条件试验显示,该菌株属中温菌,最适宜生长温度为22～35℃,且具有较宽的pH生长范围(5.0～9.0),在pH5.0时的细胞生长水平与pH9.0时基本相同,相对于已经报道的硫酸盐还原菌,其表现出较强的耐酸性.Using the Hungate anaerobic technology, was isolated from the acid sediment, The identifying a function bacteria of sulfate reducing bacteria named WF83 of morphologyical, 16Sr RNA were Carried out. This bacteria is G^-, bacillus and athletic. It can reduce the sulfate and bring out H2S. It is intersexual anaerobic. The compareability between the bacteria WF83 and Enterobacter sp. reaches to 99 %. The primary identifyication judges that it may belongs to Enterobacter sp.. The strain belongs to mid-thermophilic strain and its optimum temperature for the growth is 22-35 ℃. The strain can lives in wide pH range （5.0-9.0） and its OD when the pH 5.0 is same to that when the pH is 7.0. It is very acid-resistant relative to the sulfate-reducing bacterium have been reported which has advantages in wastewater treatment.

关 键 词：硫酸盐还原菌 鉴定 生长因素 

分 类 号：X172[环境科学与工程—环境科学]