过氧化物酶6在大鼠附睾中的表达及分布  

Expression and Distribution of Peroxiredoxin 6 in Rat Epididymidis

作　　者：王小强[1] 齐晓伟[2] 程露阳[3] 孙铁成[1] 舒如明[1] 王慧萍[4] 于和鸣[4] 

机构地区：[1]北京协和医学院研究生院,北京100730 [2]解放军总医院临床检验科,北京100853 [3]承德医学院,河北承德067000 [4]国家人口计生委科学技术研究所,北京100081

出　　处：《中国生物化学与分子生物学报》2010年第3期260-267,共8页Chinese Journal of Biochemistry and Molecular Biology

基　　金：科学技术部国家社会公益研究专项计划(No.2004DIB1J041)~~

摘　　要：通过双向电泳结合质谱技术分离鉴定正常成年大鼠附睾头段与尾段管腔液中的蛋白组成,从附睾头段及尾段管腔液的22个差异蛋白点中鉴定出12个蛋白质.其中11个蛋白质在不同种属哺乳动物的附睾组织中已有鉴定报道,而过氧化物酶6(peroxiredoxin 6,Prdx6)为新发现的存在于附睾头段及尾段管腔液中的体液蛋白.采用RT-PCR、Western印迹及免疫组化技术,对该蛋白在大鼠附睾中的表达及分布进行了分析.实验表明,Prdx6与精子的成熟、贮存及保护有一定关系,其具体机制值得进一步深入研究.This study is to separate and identify the proteins in epididymal luminal fluid from the caput and cauda segments of normal mature rat＇s epididymidis by two dimensional（2D）-electrophoresis and mass spectrum.Twelve proteins were identified from 22 differential protein spots separated from the epididymal luminal fluid of caput and cauda epididymidis.Eleven of them were reported in epididymidis of different species of mammals except the peroxiredoxin6（Prdx6）.Prdx6 was a novel epididymal protein presented in the luminal fluid of caput and cauda epididymidis.Prdx6 was further studied for expression and distribution in rat＇s epididymidis with RT-PCR,Western blot and immunohistochemistry.Results suggested that Prdx6 protein may play a role in sperm maturation,storage and protection in epididymidis.The concrete mechanism of Prdx6 is worth further investigating.

关 键 词：附睾 双向电泳 质谱 过氧化物酶6(Prdx6) 

分 类 号：Q5[生物学—生物化学]