大鼠“太溪”穴区与神经系统的节段和区域相关性研究——辣根过氧化物酶法  

Segmental and Regional Innervation of Acupoint "Taixi" (KI 3) Area in the Rat: A Horseradish Peroxidase Method Study

作　　者：蒋瑾[1] 白万柱[1] 张璐[1] 景向红[1] 晋志高[1] 

机构地区：[1]中国中医科学院针灸研究所,北京100700

出　　处：《针刺研究》2010年第1期27-31,36,共6页Acupuncture Research

基　　金：中国中医科学院自主选题研究项目(Z02024)

摘　　要：目的:用辣根过氧化物酶(HRP)神经示踪技术揭示太溪穴区与周围和中枢神经系统的相关性联系。方法:将5μL1%HRP分别注入10只雄性SD大鼠内踝尖与跟腱之间的中点凹陷处(相当于人体足少阴肾经的太溪穴)。36 h后将动物灌流固定,取出大脑、延髓、脊髓和L1-L6双侧的背根神经节,然后将各部位的组织切片进行四甲基联苯胺组织化学染色来显示HRP标记的神经元及神经纤维终末。结果:所有HRP标记均出现在示踪剂注入侧的相关神经组织中。被HRP标记的神经节细胞分布在L4-L6的背根神经节,数量依次为L5>L4>L6,而被HRP标记的运动神经元呈柱状分布在L4-L6节段脊髓前角第Ⅸ层的后外侧部和中外侧部;同时,被HRP标记的跨神经节细胞中枢端神经纤维投射集中分布在L4-L6节段脊髓后角第Ⅰ、Ⅱ层的中间部,并散在分布于薄束核的中间部。结论:应用HRP神经示踪技术证明,与"太溪"穴区相关的初级神经元传出及其中枢传入在背根节、脊髓和薄束核的对应部位分别表现为节段或区域性的联系方式。Objective Horseradish Peroxidase （HRP） To study the peripheral and central neural involvements of acupoint ＂Taixi＂ （KI 3） by using Methods Ten male SD rats were used in the present study.5 μL of 1% HRP was injected into the excavate site between the apex of medial malleolus and the Achilles tendon of the left hind foot, a corresponding site of acupoint ＂Taixi＂ （KI 3） in the human body. After 36 surviving hours, the rat＇s brain, spinal cord and dorsal root ganglia （DRG） of the lumbar segments （L） were dissected from the perfused experimental animals, and their tissue sections were examined with 3, 3＇, 5, 5＇ -tetramethyl-benzidine histochemistry for revealing HRP-labeling. Results All the HRP-labeling appeared ipsilaterally to the injection side. Numerous HRP-labeled neurons were detected in the DRGs of L4 - L6 segments with their number being L5〉 L4 〉L6. A longitudinal column of HRP-labeled motoneurons was found in the dorsolateral and mediolateral portions of the spinal cord, distributing in the lamina IX from the caudal L4 to the rostral L6. Additionally, the transganglionic HRP-labeled central projection axonal terminals were found to be dense in the central part of laminae Ⅰ-Ⅱ from L4 to the rostral L6, and to scatter in the central part of gracile nucleus. Conclusion HRP-labeled primary afferent and efferent innervating acupoint ＂Taixi＂ （KI 3） are DRGs of L4 - L6, the dorsolateral and mediolateral motoneuron columns of L4 - L6, and the centrally projecting axonal terminals of laminae Ⅰ-Ⅱ of the spinal cord and the gracile nucleus.

关 键 词：太溪穴区 神经支配 辣根过氧化物酶示踪 背根节 脊髓 

分 类 号：R224.2[医药卫生—中医基础理论]