维甲酸对小鼠T细胞增殖及骨破坏因子RANKL表达的研究  

Effects of retinoic acid on proliferation of T lymphocytes and expression of RANKL in mouse in vitro

作　　者：林晓萍[1] 魏巍[1] 周晓佳[1] Toshihisa Kawai 

机构地区：[1]中国医科大学附属盛京医院口腔科,沈阳110004 [2]Department of Immunology,The Forsyth Institute.Boston,mausa02115

出　　处：《口腔医学》2010年第3期136-139,共4页Stomatology

基　　金：辽宁省自然科学基金项目(20042062;20092139);辽宁省教育厅科研基金项目(202013124)

摘　　要：目的探讨全反式维甲酸对体外培养的小鼠T淋巴细胞的增殖及核因子-КB受体活化因子配体(RANKL)表达的调节作用及相关机制。方法体外分离Aa感染的BALB/C小鼠颈T淋巴细胞,分别加入浓度为0、1×10-8、1×10-7、1×10-6、1×10-5mol/L的全反式维甲酸,培养3 d后,取100μl上清保存在-70℃冰箱中,以备sRANKL及IL-10等细胞因子的测定;另加入100μl含有3Hthymidine(0.5μCi/孔)RPMI培养液继续培养18 h,进行T细胞3H增殖率测定。结果维甲酸处理组与非处理组相比,T细胞3H增殖率下降;上清中RANKL的表达水平下降,IL-10的表达水平增强(P<0.05),二者具有负相关性(r=-0.774,P=0.001),且呈剂量依赖性。结论维甲酸可通过上调IL-10的表达并下调T细胞上的RANKL的表达水平,抑制T细胞的免疫功能,且呈剂量依赖性。Objective To investigate the effects of all trans-retinoic acid on the proliferation of T lymphocyte cells and the expression of receptor activator of NF-KB ligand （RANKL） in mouse and analyze the potential mechanisms. Methods T cells from 2 Aa immunized BALB/c mice were isolated from cervical lymph nodes and grew in vitro. After 3 days of incubation with all trans-retinoic acid （0,1 × 10^-8, 1 × 10 ^-7 , 1 × 10 ^-6,1 × 10^-5 mol/L） induced, 100 ul supernatants of cells taken from each well in 96-well plates were removed and kept in -70 ℃ freezer for cytokine quantification by ELISA. The cells were added with 100 ul of complete RPMI containing 3H thymidine （0. 5 uCi/well） and incubated 18h at 37 ℃ for cell proliferation analysis by cintilography. SASS 11. 0 software package was used for statistical analysis. Results T cell ^3H incorporation significantly decreased, the expression of IL-10 increased. and the expression of RANKL decreased, compared with control group. And they were dose-dependent（ P 〈 0. 05）. It showed a correla- tion between them（ r = -0. 774 ,P = 0. 001 ）. Conclusions All trans-retinoic acid could inhibit proliferation and activation of T cells by up-regulating the expression of IL-10 and down-regulating the expression of RANKL,and it was dose-dependent.

关 键 词：全反式维甲酸 T细胞 伴防线放线杆菌 核因子-КB受体活化因子配体 免疫反应 

分 类 号：R739.86[医药卫生—肿瘤]