日本血吸虫卵对TNBS诱导小鼠结肠炎肠黏膜表达NOD2/CARD15的影响  

Effect of Schistosoma japonicum ova on NOD2/CARD15 during TNBS-induced colitis

作　　者：夏晨梅[1,2] 张顺财[2] 

机构地区：[1]浙江省温岭市第一人民医院消化科,浙江温岭317500 [2]复旦大学附属中山医院消化内科,上海200032

出　　处：《复旦学报（医学版）》2010年第2期162-166,共5页Fudan University Journal of Medical Sciences

摘　　要：目的研究日本血吸虫卵对2,4,6-三硝基苯磺酸(2,4,6-trinitrobenzesulfonic acid,2,4,6-TNBS)诱导小鼠结肠炎肠黏膜表达NOD2/CARD15的影响。方法实验小鼠(n=50)随机分成3组:正常对照组(n=10)、TNBS+生理盐水组(n=20)和TNBS+日本血吸虫卵组(n=20),后两组用TNBS溶液灌肠(100mg/kg)建立结肠炎模型,TNBS+日本血吸虫卵组在造模前第14天和第3天分别给予腹腔注射冰冻灭活血吸虫卵10000个(1mL冰生理盐水混悬液),TNBS+生理盐水组同时给予相同体积的冰生理盐水腹腔注射,建模后第7天处死存活小鼠,用荧光定量RT-PCR(Real time PCR)法测定结肠组织的NOD2的mRNA基因表达,Western blot方法测定结肠组织NOD2蛋白表达水平。结果TNBS+日本血吸虫卵组死亡率明显下降,结肠肉眼及组织病理炎症程度明显减轻;荧光定量RT-PCR分析显示,TNBS+生理盐水组较正常组结肠黏膜NOD2 mRNA相对表达量显著增加(P<0.01),TNBS+日本血吸虫卵组较TNBS+生理盐水组NOD2 mRNA相对表达量显著下降(P<0.05);Western blot分析显示,TNBS+生理盐水组NOD2的蛋白表达量较正常组增加了近3倍(P<0.01),TNBS+日本血吸虫卵组较TNSB+生理盐水组下降52.8%(P<0.01),差异有显著统计学意义。结论结肠炎时,黏膜NOD2/CARD15表达明显升高,日本血吸虫卵抗原可能通过下调NOD2/CARD15表达改善结肠炎症状态。Objective To investigate the effect of Schistosoma japonicum ova on the expression of intestinal NOD2/CARD15 in the mice induced by 2,4,6-trinitrobenzesulfonic acid （TNBS）. Methods Mice （n=50） in the experiment were randomly allocated into 3 groups： control group （n=10）, TNBS＋saline （n=20） and TNBS＋Schistosoma japonicum ova （n=20）. TNBS enema （100 mg/kg） was applied to the two TNBS groups in order to establish a colonitis model. Schistosoma japonicum ova was administered i.p. on the 14th and 3rd day before the instillation of haptenating agent. All mice were killed on the 7th day after colitis induction. The transcription level of NOD2 in colon tissues was measured by Real time PCR, and the expression of NOD2 protein was measured by Western blot. Results The transcription and protein levels of NOD2 in TNBS-induced mice increased statistically compared with those of the normal group （P0.05）. Compared with the TNBS-induced mice, Schistosoma japonicum ova-treated ones exhibited a statistical reduction of gene and protein expression （P0.05）. Conclusions TNBS-treated mice exhibited a statistical increased expression of NOD2/CARD15, Schistosoma japonicum ova treatment reduced the severity of experimental colitis through down-regulating NOD2/CARD15.

关 键 词：2 4 6-三硝基苯磺酸 血吸虫卵 NOD2/CARD15 炎症性肠病 小鼠 

分 类 号：R532.21[医药卫生—内科学]