人黏液表皮样癌裸鼠耐药移植瘤模型的建立及其生物学特性  

Establishment and characterization of a multidrug resistant human mucoepidermoid carcinoma xenograft model

作　　者：徐小方[1] 刘斌[1] 吴军正[1] 王春梅[2] 王哲[3] 

机构地区：[1]第四军医大学口腔医学院生物学教研室,西安710032 [2]第四军医大学中心实验室 [3]第四军医大学口腔医学院实验动物中心

出　　处：《实用口腔医学杂志》2010年第2期177-180,共4页Journal of Practical Stomatology

基　　金：国家自然科学基金资助(编号:30470471;编号:30672343)

摘　　要：目的:建立一种耐药性稳定的人涎腺黏液表皮样癌裸鼠移植瘤模型。方法:将人涎腺黏液表皮样癌多药耐药细胞接种于裸鼠皮下,成瘤后经腹腔注射小剂量5-FU进行体内诱导,建立稳定的黏液表皮样癌裸鼠移植瘤模型。结果:裸鼠移植瘤原代培养的MEC/5-FU/NU细胞与体外培养细胞结构类似,MEC/5-FU/NU细胞对5-FU的耐药指数为27.82。RT-PCR显示耐药相关基因ABCB1、ABCB11、GSTA1在MEC/5-FU/NU细胞中明显高表达(P<0.05)。免疫荧光显示多药耐药蛋白MDR-1在MEC/5-FU/NU细胞中高表达。结论:以MEC/5-FU建立的黏液表皮样癌裸鼠皮下移植瘤模型仍保持MEC/5-FU细胞的形态学特征和多药耐药性,可为耐药性人涎腺黏液表皮样癌耐药机制和逆转的研究提供较理想的动物模型。Objective： To establish a multidrug resistant model of human mucoepidermoid carcinoma using nude mice. Methods： Multidrug resistant MEC/5-FU cells were inoculated intradermally into nude mice. Solid tumors were locally measurable after 10 days and 5-FU was repeated intraperitoneal injected into tumor-bearing mice. The tumor cells in nude mice （MEC/5-FU/NU） were isola- ted, cultured and examined. Results：The xenografts were similar to the original mucoepidermoid carcinoma from which the cell line was derived. The resistance index （RI） of the MEC/5-FU/NU cells to 5-FU was 27.82. Compared to the MEC, the expressions of ABCB1, ABCBll and GSTA1 genes and MDR-1 protein increased in the MEC/5-FU/NU cells（P 〈0.05）. Conclusion：The xenograft model is a good model of human multidrug resistant mucoepidermoid carcinoma, and may be useful in studying drug resistance mechanism in vivo.

关 键 词：黏液表皮样癌 多药耐药 裸鼠 

分 类 号：R739.8[医药卫生—肿瘤]