富血小板血浆对牙周膜成纤维细胞的增殖、迁移和分化的影响  

Effect of platelet-rich plasma on human periodontal ligament fibroblasts' proliferation,migration and differentiation

作　　者：施六霞[1,2] 狄昌萍[1,2] 徐艳[1,2] 李璐[1,2] 王晓茜[1,2] 

机构地区：[1]南京医科大学口腔医学研究所,210029 [2]南京医科大学附属口腔医院牙周科

出　　处：《实用口腔医学杂志》2010年第2期194-197,共4页Journal of Practical Stomatology

基　　金：江苏省卫生厅科研项目(编号:H200752)

摘　　要：目的:体外研究不同浓度的富血小板血浆(platelet-rich plasma,PRP)对牙周膜成纤维细胞(periodontal ligamentfibroblasts,PDLFs)的增殖、迁移和分化的影响。方法:不同浓度PRP(10,50,100,200,300,500ml/L),加入到原代培养的人PDLFs,培养时间为3、5、7d,MTT法测定细胞增殖效果,transwell系统测定细胞迁移效果,AKP试剂盒测定碱性磷酸酶活性。结果:PRP有明显促进增殖作用,以200ml/L浓度PRP效果最好,更高浓度促进作用反而减低。细胞迁移效果中,各浓度组均比阴性对照组效果好,尤其是100ml/L的效果最佳。对于碱性磷酸酶活性,也具有明显的促进作用,以300ml/L的效果最佳。结论:PRP在一定浓度范围内对人PDLFs功能有明显促进效果,但是在更高浓度下,这种促进效果反而减低。Objective： To investigate the in vitro effects of platelet-rich plasma（PRP） on human periodontal ligament fibroblasts （PDLFs）. Methods： Various concentrations of PRP （10, 50, 100, 200, 300, 500 ml/L） were applied to primary cultures of human PDLFs. MTT assays were utilized to assess cell proliferation ability. Migration was determined by assessing the cell response to a concentration gradient with Transwell chamber. Differentiation was assessed using alkaline phosphatase （ALP） kit. Results： A beneficial effect on proliferation was observed, especially in response to 200 ml/L PRP. PRP had stimulatory effects on the migration of human PDLFs. PRP facilitated differentiation of PDLFs. Conclusion： PRP can exert a positive effect on human PDLFs,but this effect is concentration specific, while higher concentrations is not necessary to result in optimal outcomes.

关 键 词：牙周膜成纤维细胞 富血小板血浆 TRANSWELL 

分 类 号：R78[医药卫生—口腔医学]