唾液支原体诱导牙龈上皮细胞产生TNF-α和IL-6  

作　　者：王玉爱[1] 刘志杰[1] 

机构地区：[1]湖南怀化医学高等专科学校医学检验系,418000

出　　处：《当代医学》2010年第12期18-19,共2页Contemporary Medicine

摘　　要：目的了解唾液支原体能否诱导牙龈上皮细胞(HGEC)产生细胞因子TNF-α和IL-6。方法培养HGEC上皮细胞,用唾液支原体感染后,ELISA检测TNF-α和IL-6的产生情况,同时用核因子κB特异性抑制剂PDTC处理HGEC后,研究其在TNF-α和IL-6中产生的作用。结果唾液支原体能以时间依赖性方式诱导HGEC产生TNF-α和IL-6,并且PDTC能部分抑制其功能。结论唾液支原体诱导HGEC产生TNF-α和IL-6,这可能是其致病的机制之一,其机制可能与核因子κB激活有关。Objective This study was designated to investigate production of TNF-α and IL-6 in gingival epithelium cells （HGEC） induced by Mycoplasma salivarium.Methods Cultured HGEC cells were infected by M.salivarium,and TNF-α and IL-6 were detected by ELISA.NF-κB specifi c inhibitor PDTC was used to assess it＇s role in the production of cytokines.Results M.salivarium could induce HGEC cells to product TNF-α and IL-6 in time-dependent manner,and PDTC could abrogate it.Conclusion Production of TNF-α and IL-6 might related to the pathogenesis of M.salivarium,NF-κB may also involved

关 键 词：唾液支原体 牙龈上皮细胞 TNF-Α IL-6 

分 类 号：R78[医药卫生—口腔医学]