DAPI染色对A549肺癌细胞弹性模量的影响  

Effect of DAPI Stain on Elastic Properties of A549 Cells

作　　者：叶志义[1] 张丽[1] 范霞[1] 

机构地区：[1]重庆大学生物工程学院,"生物流变科学与技术"教育部重点实验室(重庆大学),重庆400044

出　　处：《中国细胞生物学学报》2010年第1期137-140,共4页Chinese Journal of Cell Biology

基　　金：国家自然科学基金项目(No.30570453);高等学校学科创新引智计划("111")(B06023)资助~~

摘　　要：原子力显微镜(AFM)能测量活细胞的力学性质。DAPI染色A549肺癌细胞核后用AFM测量细胞的弹性模量,分析DAPI染色对细胞力学性质的影响。通过Hertz模型拟合AFM测量压痕细胞的力-压痕曲线,得到细胞的弹性模量。实验结果:用DAPI染色细胞后其弹性模量为3.66±2.08 kPa,而对照组未染色的细胞弹性模量为3.21±1.73 kPa,数据比较显示,DAPI染色对A549肺癌细胞弹性模量没有显著性影响。Mechanical properties of living cell can be determined by using atomic force microscopy （AFM）. In this study, the A549 cells line and A549 cell line which was dyed by DAPI were measured by AFM. Here we report the effect of DAPI on the A549 cell mechanical properties. Cells were incubated with DAPI, and individual cell stiffness was tracked with AFM. We used AFM to measure quantitatively the local mechanical properties of A549 cells. To get quantitative information on sample elasticity, forces vs. indentation curves were got. The curves were then fitted with the Hertz model, yielding Young＇s modulus values of 3.21±1.73 kPa and 3.66±2.08 kPa for the A549 cell and similar cell dyed by DAPI, respectively. There was no significant difference between the A549 cell and the similar cell dyed by DAPI stain. These observations suggest that DAPI will not lead to the change of cell＇s stiffness.

关 键 词：原子力显微镜 弹性模量 DAPI染色 

分 类 号：R734.2[医药卫生—肿瘤]