肺腺癌患者血清CEA和蛋白质谱联合检测的临床意义  

Clinical significance of combined application of patients CEA detection and SELDI-TOF-MS technology in lung adenocarcinoma patients

作　　者：林艳[1] 林志强[2] 陈慧菁[1] 叶韵斌[1] 林丽婷[2] 陈强[1] 

机构地区：[1]福建医科大学教学医院(福建省肿瘤医院)肿瘤免疫学研究室,福建福州350014 [2]福建中医学院附属人民医院检验科,福建福州350004

出　　处：《中华肿瘤防治杂志》2010年第3期215-217,共3页Chinese Journal of Cancer Prevention and Treatment

基　　金：福建省自然科学基金计划资助项目(X0750024)

摘　　要：目的:探讨在肺腺癌患者中联合应用CEA检测和SELDI蛋白芯片技术诊断的价值。方法:应用免疫化学发光技术检测69例肺腺癌患者及71例健康对照者血清标志CEA;同时应用表面增强激光解吸电离飞行时间质谱技术(SELDI-TOF)和CM10芯片检测该血清,经BiomarkerWizard软件分析,结合临床病理资料分析两种手段的联合诊断价值。结果:免疫化学发光法检测CEA的敏感度为62.319%(43/69),特异度为92.958%(66/71);SELDI-TOF-MS技术检测的敏感度为88.406%(61/69),特异度为68.182%(15/22);两者联合检测的敏感度为82.609%(57/69),特异度为92.958%(66/71)。结论:免疫化学发光法检测CEA和SELDI-TOF-MS技术联合检测蛋白标志比单用免疫化学发光法检测CEA能提高对肺腺癌诊断的敏感度。但与单独使用SELDI-TOF-MS技术相比,两者联合检测的敏感度无明显提高。OBJECTIVE：To study the diagnostic values of co-detections of traditional serum tumor marker,carcino-embryonic antigen （CEA） and serum proteomic patterns by surface-enhanced laser desorption and ionization time-of-flight mass spectrometry （SELDI-TOF-MS） technology in patients with lung adenocarcinoma.METHODS：The serum samples of 69 lung adenocarcinoma patients and 71 healthy controls were detected by immunoluminesceme to analyze the levels of CEA,while SELDI-TOF-MS and CM10-chips were employed to detect the differential expressions of proteomes in serum,followed by Biomarker Wizard software analysis.Combined with clinical pathological materials,the joint diagnostic values of the two methods were evaluated.RESULTS：The sensitivity and specificity of CEA detection were 62.319%（43/69） and 92.958%（66/71）,while SELDI-TOF-MS technology were 88.406%（61/69） and 68.182%（15/22）.Meanwhile,combined detections of the two methods were 82.609%（57/69） and 92.958%（66/71）,respectively.CONCLUSION：The sensitivity of immunoluminescence for CEA detection together with the SELDI-TOF-MS technology is higher than that of immunoluminescence for CEA detection alone in the diagnosis of lung adenocarcinoma,but it is not up-regulated obviously compared with SELDI-TOF-MS technology.

关 键 词：肺肿瘤 腺癌 血清 癌胚抗原 

分 类 号：R734.2[医药卫生—肿瘤]