青冈栎果实抗癌活性的实验研究  

Experimental study on anti-cancer activity of the fruit Cyclobalanopsis glauca

作　　者：甘耀坤[1] 陈旭健[1] 韦敏[2] 叶楚芳[1] 卿莉萍[1] 

机构地区：[1]玉林师范学院化学与生物系,玉林537000 [2]玉林市卫生学校,玉林537000

出　　处：《食品科技》2010年第3期227-229,共3页Food Science and Technology

基　　金：广西科技厅自然基金项目(桂科自0728250);广西教育厅科研项目(教育厅200709LX140);广西教育厅科研项目(教育厅200810LX403)

摘　　要：目的:研究青冈栎果实提取液的抗癌活性,探讨青冈栎的抗癌机理。方法:在体外培养的胃癌细胞(MGC-803)、子宫颈癌细胞(hela)和肝癌细胞(BEL-7404)中分别采用浓度为0.40、0.81、1.61、3.13、6.25、12.50、25.00mg/mL的青冈栎果实提取液处理,然后利用四甲基偶氮唑蓝法(MTT比色法)定量测定肿瘤细胞的生长抑制情况。结果:青冈栎果实提取液浓度在0.81～25.00mg/mL时对上述3种癌细胞均有抑制效果,其中在25mg/mL浓度下抑制效果最好,对MGC-803、hela和BEL-7404的抑制率分别是72.85%、76.64%、71.81%。结论:青冈栎果实提取液在一定浓度下能抑制体外培养的MGC-803、hela和BEL-7404细胞的生长,显示青冈栎中含有抗癌的有效成分。Objective：To study Cyclobalanopsis glauca fruit extractive anti-cancer activity,to explore the anticancer mechanism of Cyclobalanopsis glauca.Methods：In vitro cultivation of cells were used in 0.40,0.81,1.61,3.13,6.25,12.50,25 mg/mL density of Cyclobalanopsis glauca fruit extractive and then use MTT method （MTT colorimetric） quantitative measurement of tumor cell growth inhibition of the situation.Results：Cyclobalanopsis glauca fruit extractive in 25 mg/mL concentration of gastric cancer cells show （MGC-803）,cervical cancer（hela）,liver cancer cells（BEL-7404） higher inhibitory rate：72.85%,76.64%,71.81%.Conclusion：Cyclobalanopsis glaua fruit extractive can be selective inhibition of gastric cancer cells in vitro （MGC-803）,liver cancer cells（BEL-7404）,cervical cancer （hela） Growth at a certain concentration,it shows that Cyclobalanopsis glauca contain the active ingredient in anti-cancer.This research could be provided a theoretical basis for the further development and utilization of health care to develop anti-cancer drugs of the Russula fruiting bodies and Cyclobalanopsis glauca.

关 键 词：青冈栎 果实 抗癌活性 

分 类 号：R284.2[医药卫生—中药学]