稀释液中分别添加不同浓度咖啡因或谷胱甘肽对犬精液冷冻效果的影响  

Effect of Different Concentrations of Caffeine and Glutathione in the Basic Frozen Semen Dilution on Quality of Frozen Dog Sperm

作　　者：林自力[1] 何艳芳[1] 卢婷婷[1] 康蕊[1] 秦秀蕊[1] 张鑫[1] 倪和民[1] 

机构地区：[1]北京农学院动物科学技术系,北京102206

出　　处：《中国农学通报》2010年第8期21-24,共4页Chinese Agricultural Science Bulletin

基　　金：国家"十一五""863计划"重点项目"家畜卵泡高效诱导发育技术研究与应用"(2008AA101003);国家"十一五科技支撑计划"项目"肉羊高效育种关键技术的研究与应用"(2008BADB2B04-4);2008年度北京市大学生科学研究与创业行动计划项目"几种主要家养动物精子获能方法的比较"

摘　　要：研究在冷冻稀释液中分别添加不同浓度咖啡因或谷胱甘肽对犬精液冷冻效果的影响,以优化犬精液冷冻方案,提高解冻后精子质量。在犬的常规精液冷冻稀释液中分别添加不同浓度咖啡因和谷胱甘肽,经细管冷冻,解冻后通过分别评价其精子的活率、形态(包括顶体完整率和畸形率等)等来筛选其最佳添加浓度。经解冻后的精液质量评价如下:(1)向稀释液中添加5mmol/L咖啡因对解冻后精子活率明显改善,为0.33±0.04(P<0.01),显著高于其他组;当添加浓度为12.5mmol/L时,活率仅为0.230±0.04(P<0.01),显著低于对照组;添加浓度为5mmol/L时精子畸形率最低,为0.174±0.036(P<0.01),较其他组差异显著。(2)向稀释液中添加谷胱甘肽5mmol/L,精子活率为0.31±0.054,顶体完整率为0.438±0.022,畸形率仅为0.162±0.025,均优于其他浓度组,差异显著(P<0.01)。在犬的精液冷冻液中分别添加适量浓度的咖啡因或谷胱甘肽均对冷冻效果有显著的促进作用。This experiment was aimed to improve the freeze-thawing effect of the dog’s sperm, and detect the effect of caffeine and glutathione in the basic semen dilution on guality of frozen dog sperm. Method： The Caffeine or glutathione was added into the routine semen dilution and then, the quality of frozen dog sperm was described by the following three aspects： spermatozoa progressive motility, sperm deformity rate and acrosome intactness rate. Results： After post-thawing, the quality of frozen dog semen was evaluated as following： （1） The sperm livability in the group added with 5mmol / L of caffeine was significantly higher than that of the other groups （P 〈0.01）, and the sperm abnormality rate was significantly lower than that in the other groups（P 〈0.01）, but while it was added with 12.5mmol / L of caffeine, the appropriate data was significantly lower than that in the control one（P 〈0.01）. （2） The sperm livability, acrosome integrity rate, and malformation rate in the group added with 5mmol / L of glutathione, were all significantly better than that in the others （P 〈 0.01）. Conclusion： It can be concluded that the appropriate supplement of caffeine and glutathione in the basic frozen semen dilution may improve the quality of dog sperm through their normal frozen-thawing processes.

关 键 词：犬 冷冻精液 冻精稀释液 咖啡因 谷胱甘肽 

分 类 号：S882[农业科学—特种经济动物饲养]