琥珀酰亚胺类固定化酶载体的合成与胺解研究  

Studies on Synthesis and Aminolysis of Succinimide Immobilized Enzyme Carrier

作　　者：蔡莉[1] 马骏[1] 粟慧君[1] 廖洋[1] 马玉洁[1] 赵仕林[1] 

机构地区：[1]四川师范大学化学与材料科学学院,四川成都610066

出　　处：《四川师范大学学报（自然科学版）》2010年第2期224-227,共4页Journal of Sichuan Normal University（Natural Science）

基　　金：四川省科技攻关项目(05JY029-129)资助项目

摘　　要：选用丙烯酸(AA)、N′-羟基琥珀酰亚胺(NHM)为单体,以N′,N′-亚甲基双丙烯酰胺(MBAA)为交联剂,以乙醇水溶液为制孔剂条件下进行反相悬浮聚合,得到珠状共聚物载体,该载体是一种新的酶载体形式,在国内外的相关文献中均未见报道.用红外光谱对其结构进行了表征.对载体与有机胺的胺解反应进行了研究,发现共聚物载体分子链上的丙烯酸-N′-羟基琥珀酰亚胺酯基易于在温和条件下与胺基发生胺解反应,从而为该类载体应用于含游离胺基的生物活性物质分子的固定化提供了依据.Taking acrylic acid（AA） and N′-hydroxysuccinimide as monomer,N′,N′-methylene-bis（acrylamide）（MBAA） as crosslinking agent,and ethanol solution as porosity preparation,a new copolymer carrier has been obtained by inverse suspension polymerization,a new copolymer carrier has been obtained by inverse suspension polymerization.This carrier has not been reported in the literatures.The support structure is characterized by infrared spectroscopy.The aminolysis reaction of copolymer carriers with compounds containing amino group is investigated,and the results show that the ester group of copolymer carriers from N′-hydroxysuccinimide could react with amino group easily in mild conditions,for such copolymer carriers could be used in immobilization of bioactive substance molecules containing free amino group.

关 键 词：聚合物载体 反向悬浮聚合 固定化酶 胺解 

分 类 号：O633.22[理学—高分子化学]