小鼠脑缺血再灌注模型的构建及条件优化  

Establishment and optimization of mice brain ischemia Reperfusion model

作　　者：王梧霖[1,2] 史小军[2] 侯天德[1] 米锡阳[2] 

机构地区：[1]西北师范大学生命学部,甘肃兰州730000 [2]清华大学深圳研究生院生命科学与海洋生物学实验室,广东深圳518055

出　　处：《现代生物医学进展》2010年第1期70-73,共4页Progress in Modern Biomedicine

基　　金：深圳市国际科技合作资助计划项目(ZYA200903270135A)

摘　　要：目的:构建小鼠的脑缺血再灌注模型,并对其进行优化。方法:通过对线栓法缺血再灌注脑损伤模型的各个因素的对比实验,在线拴位置、线径、进线深度、小鼠种类等方面对该方法进行了优化改进。结果:造模后的脑片经TTC染色,梗死区域清晰;神经功能评分的体征明显。现有条件下构建的小鼠MCAO模型,成功率可以达到80%以上,成活率可以达到90%以上。与对照组相比,小鼠MCAO模型组血清SOD活性及MDA水平有显著变化(P<0.01)。结论:经改进和优化,小鼠脑缺血再灌注模型的成功率和成活率大大提高且症状明显,对缺血缺氧性神经损伤研究具有一定的参考价值。Objective：Establish and optimize the mice brain ischemia reperfusion model（also known as MCAO model）.Methods：Factors of this model,such as the position of line,the diameter of line,the depth of line and mice type,were altered to optimize ischemia-reperfusion brain damage model using mice brain ischemia reperfusion model as comparative experiments.Results：The brain slices of improved model were found clear infarction area and obvious neurobehavioral scores of signs after TTC dyeing.The livability could reach 80% under existing MCAO model conditions,whereas,the survival rate of improved model achieved more than 90%.Meanwhile,there were significant change of MDA and SOD activity in serum of the improved MCAO model（P0.01）,compared with the control group.Conclusion：The higher success rate,survival rate and clear symptoms were observed after the improvement and optimization of cerebral ischemia reperfusion.The improved model provide certain reference value for research on oxygen deficiency nerve injury.

关 键 词：小鼠 脑缺血再灌注模型 优化 

分 类 号：Q95-3[生物学—动物学] R743.31[医药卫生—神经病学与精神病学]