蜗牛酶转化淫羊藿苷制备淫羊藿苷元的研究  

Study on preparation of icaritin by enzymolysis of icariin with snail hydrolase

作　　者：贾东升[1,2] 贾晓斌[1,2] 薛璟[1] 施峰[1] 孙娥[1] 陈玲玲[1] 张振海[1] 

机构地区：[1]江苏省中医药研究院中药新型给药系统重点实验室,国家中医药管理局中药口服制剂释药系统重点研究室,江苏南京210028 [2]江苏大学药学院,江苏镇江212013

出　　处：《中国中药杂志》2010年第7期857-860,共4页China Journal of Chinese Materia Medica

基　　金：江苏省医药高技术计划项目(BG2007614);江苏省中医药领军人才项目(2006)

摘　　要：目的:研究淫羊藿苷元(IT)的制备工艺。方法:采用蜗牛酶水解淫羊藿苷制备IT,以转化率为指标,通过单因素考察pH、温度、底物的浓度、酶用量、反应时间及金属离子对转化率的影响,正交试验优化制备工艺;采用MS,1H-NMR,13C-NMR鉴定水解产物。结果:酶解反应的最适条件为温度37℃、反应介质pH6.0醋酸-醋酸钠缓冲液,底物质量浓度为10 g.L-1,酶与底物比为1∶1,反应时间48 h,Na+,Ca2+,Mg2+和Zn2+对酶解反应无显著影响,Fe2+对酶解反应有抑制作用(P<0.01);反应产物相对分子质量为368,核磁图谱证实产物为IT。结论:蜗牛酶水解淫羊藿苷制备IT反应条件温和,工艺简单可靠,适合工业化生产。Objective： To optimize the conditions for enzymolysis of Icariin by Snail Hydrolase. Methods： Take conversion as index, the effects of pH, temperature, reaction time, dosage of enzyme, concentration of Icariin and metal ion on hydrosis were studied by Single-factor designs and a L9 （34） Orthogonal design. The product was characterized through MS, 1H-NMR and 13C-NMR methods. Results： The optimum enzymolysis conversion was achieved at 37℃, pH6.0 acetic acid - sodium acetate buffer solution and 48 h. The Ca2＋, Mg2＋ and Zn2＋ could active helicase slightly but the Fe2＋ inhibite enzymolysis significantly. The production was presumed as icaritin on the basis of spectral evidences. Conclusions： Snail hydrolase can be used for preparing of IT. The condition is mildness and suitable for industrialization.

关 键 词：蜗牛酶 淫羊藿苷 IT 生物转化 

分 类 号：R284.1[医药卫生—中药学]