厚朴枝中厚朴酚及和厚朴酚提取工艺比较  

Comparative Study of Different Extraction Techniques for Extraction of Magnolol and Honokiol from Branches of Megnolia Hypoleuca

作　　者：张朝晖[1] 王秋兰[1] 姚茂君[1] 

机构地区：[1]吉首大学化学化工学院,湖南吉首416000

出　　处：《食品科学》2010年第6期32-35,共4页Food Science

基　　金：湖南省自然科学基金项目(08JJ4004);湖南省教育厅优秀青年基金项目(07B061)

摘　　要：采用加热回流提取法、超声波辅助提取法、微波辅助萃取法和超临界CO2流体提取等技术从厚朴枝中提取厚朴酚及和厚朴酚,优化提取条件,用反相高效液相色谱法(RP-HPLC)测定厚朴酚及和厚朴酚的提取得率,比较不同提取方法对厚朴枝中厚朴酚及和厚朴酚的提取效果。结果表明,微波辅助提取厚朴枝中厚朴酚速度最快,当微波功率为低火(1～2.8PV,200W)、乙醇体积分数65%、料液比1:40(mg/mL)、提取时间10min时,单次提取得率最高,厚朴酚与和厚朴酚及总酚得率分别为7.893mg/g和1.938mg/g。Heat reflux,ultrasonic-assisted extraction,microwave-assisted extraction and supercritical carbon dioxide extraction were compared for extraction of magnolol and honokiol from branches of Megnolia Hypoleuca.Reverse-phase HPLC was used for quantitative analysis of magnolol and honokiol.Results showed that among four extraction techniques,the least time was consumed for the microwave-assisted extraction of magnolol and honokiol,and the single microwave-assisted extraction for 40 min at 200 W（1-2.8 PV） with 65% ethanol as extraction solvent using a material/liquid ratio of 1：40（mg/mL） gave the highest yields of magnolol and honokiol,which were valued at 7.893 mg/g and 1.938 mg/g,respectively.

关 键 词：厚朴酚 和厚朴酚 提取工艺 高效液相色谱 

分 类 号：R284.2[医药卫生—中药学]