罗格列酮对断奶仔猪肠黏膜结构和抗氧化能力的影响  

Effect of Rosiglitazone on Intestinal Morphology and Antioxidant Ability in Weaned Pigs

作　　者：刘玉兰[1] 范伟[1] 孟国权[1] 车政权[1] 朱惠玲[1] 侯永清[1] 丁斌鹰[1] 

机构地区：[1]武汉工业学院动物营养与饲料科学湖北省重点实验室,农副产品蛋白质饲料资源教育部工程研究中心,湖北武汉430023

出　　处：《中国畜牧杂志》2010年第3期15-19,共5页Chinese Journal of Animal Science

基　　金：国家自然科学基金面上项目(30500362和30972109);教育部科学技术研究重点项目(209082)

摘　　要：试验研究罗格列酮(ROS)对脂多糖(LPS)刺激的断奶仔猪肠黏膜结构和抗氧化能力的影响。试验包括3个处理组,对照组注射生理盐水,LPS组注射100μg/kg体重的LPS,ROS组在注射LPS之前的30min注射3mg/kg体重的ROS。注射LPS3h后取样。结果表明:ROS提高了十二指肠和回肠DNA的含量(P<0.05);ROS缓解了LPS导致的十二指肠、空肠和回肠隐窝深度的增加(P<0.10)和绒毛高度/隐窝深度的降低(P<0.01);ROS降低了空肠丙二醛含量(P<0.10),提高了十二指肠和空肠SOD活性(P<0.05)。结果表明,ROS可能是通过提高肠道抗氧化功能而对LPS刺激的断奶仔猪小肠形态结构的损伤起到一定保护作用。This experiment was conducted to determine the effect of rosiglitazone（ROS） on intestinal morphology and antioxidant ability in weaned pigs after Escherichia coli lipopolysaccharide（LPS） challenge.Piglets were allotted to three treatments including：control group;LPS-challenged group（LPS）;ROS ＋ LPS group（ROS）.Pigs were injected with 100 μg/kg BW of LPS or sterile saline.ROS or its vehicle was given 30 min before LPS injection.At 3 h following injection of LPS,pigs were sacrificed and small intestin samples were collected.The results showed that ROS increased DNA content in duodenum and ileum（P〈0.05） compared to LPS pigs.ROS alleviated LPS-induced intestinal morphology disorder,which was manifested as lower crypt depth（P〈0.10） and higher villus height：crypt depth ratio（P〈0.01） in duodenum,jejunum and ileum.ROS decreased malonaldehyde content in jejunum（P〈0.10）,and increased SOD activity in duodenum and jejunum（P〈0.05）.These results indicated that ROS has beneficial effects in alleviating intestinal morphology damage induced by LPS challenge,which may be associated with increasing intestinal mucosal antioxidant ability.

关 键 词：罗格列酮 脂多糖 肠黏膜结构 抗氧化能力 仔猪 

分 类 号：S828.5[农业科学—畜牧学]