Biodegradation of phenol and m-cresol by mutated Candida tropicalis  

作　　者：Yan Jiang Xun Cai Di Wu Nanqi Ren 

机构地区：[1]State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China. [2]Department of Chemical Engineering, Daqing Oilfield Engineering Limited Company, Daqing 163712, China [3]School of Life Sciences and Chemistry, Harbin University, Harbin 150016, China

出　　处：《Journal of Environmental Sciences》2010年第4期621-626,共6页环境科学学报（英文版）

基　　金：supported by the Youth Academic Cadreman Project of Heilongjiang Provincial Education Department (No.1152G068);the Natural Science Foundation of Heilongjiang Provincial (No.B200819);the 41st Science Fund of China Postdoctor (No.20070410268)

摘　　要：The phenol and m-cresol biodegradations were studied using the mutant strain CTM 2 obtained by the He-Ne laser irradiation on wild-type Candida tropicalis. The results showed that C. tropicalis exhibited the increased capacity of phenolic compounds degradation after laser irradiation. It could degrade 2600 mg/L phenol and 300 mg/L m-cresol by 5% inoculum concentration, respectively. In the dual-substrate biodegradation system, 0-500 mg/L phenol could accelerate m-cresol biodegradation, and 300 mg/L m-cresol could be completely utilized within 46 hr at the presence of 350 mg/L phenol. Besides, the maximum biodegradation of m-cresol could reach 350 mg/L with 80 mg/L phenol within 61 hr. Obviously, phenol, as a growth substrate, could promote CTM 2 to degrade m-cresol, and was always preferentially utilized as carbon source. Comparatively, low-concentration m-cresol could result in a great inhibition on phenol degradation. In addition, the kinetic behaviors of cell growth and substrate biodegradation were described by kinetic model proposed in our laboratory.The phenol and m-cresol biodegradations were studied using the mutant strain CTM 2 obtained by the He-Ne laser irradiation on wild-type Candida tropicalis. The results showed that C. tropicalis exhibited the increased capacity of phenolic compounds degradation after laser irradiation. It could degrade 2600 mg/L phenol and 300 mg/L m-cresol by 5% inoculum concentration, respectively. In the dual-substrate biodegradation system, 0-500 mg/L phenol could accelerate m-cresol biodegradation, and 300 mg/L m-cresol could be completely utilized within 46 hr at the presence of 350 mg/L phenol. Besides, the maximum biodegradation of m-cresol could reach 350 mg/L with 80 mg/L phenol within 61 hr. Obviously, phenol, as a growth substrate, could promote CTM 2 to degrade m-cresol, and was always preferentially utilized as carbon source. Comparatively, low-concentration m-cresol could result in a great inhibition on phenol degradation. In addition, the kinetic behaviors of cell growth and substrate biodegradation were described by kinetic model proposed in our laboratory.

关 键 词：BIODEGRADATION the mutant strain CTM 2 PHENOL M-CRESOL KINETIC 

分 类 号：TQ243.1[化学工程—有机化工] X172[环境科学与工程—环境科学]