细胞外基质蛋白促晶状体上皮细胞粘附的作用  

In vitro study on the promotion of adhesion of lens epithelial cells by fibronectin,laminin and type I collagen

作　　者：吴强[1,2] 陈才根[1,2] 王丽天[1,2] 吴念祖[1,2] 

机构地区：[1]上海第二医科大学附属新华医院眼科 [2]上海第六人民医院眼科

出　　处：《眼科研究》1998年第4期259-261,共3页Chinese Ophthalmic Research

摘　　要：目的探讨纤粘连蛋白、层粘连蛋白、Ⅰ型胶原３种细胞外基质蛋白促牛晶状体上皮细胞（ＬＥＣ）的粘附作用，从细胞生物学方面阐述后囊膜混浊（ＰＣＯ）发生的病理学机制。方法应用３Ｈ—ＴＤＲ掺入法研究纤粘连蛋白、层粘连蛋白、Ｉ型胶原和鸡卵白蛋白对体外培养的牛ＬＥＣ的粘附作用。结果牛ＬＥＣ能够粘附到包被有不同浓度的纤粘连蛋白、层粘连蛋白、Ⅰ型胶原培养板表面上，呈现出浓度和时间效应特点；但牛ＬＥＣ不能够粘附到包被有不同浓度的鸡卵白蛋白培养板表面上。结论纤粘连蛋白、层粘连蛋白、Ⅰ型胶原能够介导ＬＥＣ粘附到后囊膜上，在ＰＣＯ发生过程中它们可起到重要的促进作用。ObjectiveTo inquire into the role of fibronectin,laminin and type I collagen in promotion of bovine lens epithelial cells(LEC)adhesion and to explain the pathogenesis of posterior capsular opacification(PCO).Methods 3H TDR incorporating assays were applyed to study adhesion of bovine LEC cultued in response of fibronectin,laminin,type I collagen and ovalbumin in vitro.ResultsBovine LEC adhered to surfaces coated with varying concentrations of fibronectin,laminin and type I collagen,and the adhesion of the cells was dependent on the concentration of coated substates and time.Bovine LEC did not adhere to ovalbumin coated surfaces at any concentration tested.ConclusionThe results of these studies indicate that adhesion of LEC occurs in response to fibronectin,laminin and type I collagen,which may play a critical promotive role in the occurrence of PCO.

关 键 词：晶状体上皮细胞 细胞外基质蛋白 LEC PCO 

分 类 号：R776.02[医药卫生—眼科]