藏猪背最长肌中过氧化物酶体增殖物激活受体γ辅激活因子1α基因表达的发育性变化  

Developmental Expression Changes of PPARGC1A Gene in Longissimus Dorsi Muscle of the Tibetan Pigs

作　　者：蒋岸岸[1] 李明洲[1] 顾以韧[1] 张凯[1] 李学伟[1] 

机构地区：[1]四川农业大学动物科技学院,四川雅安625014

出　　处：《中国畜牧杂志》2010年第5期1-3,共3页Chinese Journal of Animal Science

基　　金：国家公益性行业(农业)科研专项(NYHYZX07-034);国家现代农业产业技术体系专项(NYCYTX-009)

摘　　要：采用实时荧光定量PCR方法检测了藏猪背最长肌组织中过氧化物酶体增殖物激活受体γ辅激活因子1α(PPARGC1A)基因mRNA表达量在2、4、6月龄和8月龄间的差异。结果表明:藏猪背最长肌中PPARGC1A基因mRNA的表达量在2月龄时最低,至4月龄时表达量极显著上升(P<0.01),6月龄时表达量逐渐下降,但较4月龄差异不显著(P>0.05),至8月龄时表达量又极显著上升到最高水平(P<0.01)。本研究初步揭示了藏猪PPARGC1A基因的发育表达模式,为进一步研究PPARGC1A基因对猪肉质性状的影响提供基础数据。The expression changes of peroxisome proliferators activated receptor γ coactivator 1α （PPARGC1A） gene in longissimus dorsi muscle of Tibetan pigs at four postnatal growth stages （2,4,6 and 8 months） were measured by using real-time quantitative PCR approach.The results indicated that the mRNA abundance of PPARGC1A gene was lowest at 2-month,then strongly up-regulated to a higher level at 4-month （P〈0.01）.After a slight down-regulation during 4-to 6-month （P〈0.05）,the highest mRNA abundance was observed at 8-month（P〈0.01）.These results highlight PPARGC1A as a possible candidate genes for pork quality traits and provide some data on which to base further study of the molecular basis of skeletal muscles growth.

关 键 词：背最长肌 PPARGC1A 荧光实时定量PCR 猪 

分 类 号：S828.2[农业科学—畜牧学]