中国人肥胖基因的克隆、序列分析及在大肠杆菌中的表达  

The molecular cloning,sequence analysis and high efficiency expression in E.coli of Chinese obese gene

作　　者：周炜[1,2] 缪为民[1,2] 周丙荣[1,2] 王立明[1,2] 陈志辉[1,2] 焦炳华[1,2] 

机构地区：[1]第二军医大学微生物学教研室 [2]复旦大学遗传学研究所遗传工程国家重点实验室

出　　处：《中华内分泌代谢杂志》1998年第4期228-231,共4页Chinese Journal of Endocrinology and Metabolism

摘　　要：目的克隆中国人肥胖基因，并进行序列分析和在大肠杆菌中表达。方法采用ＲＴＰＣＲ法从中国人脂肪组织ＲＮＡ中扩增出肥胖基因（Ｏｂｅｓｅｇｅｎｅ），进行核苷酸顺序分析，并将该基因克隆入原核表达载体，在大肠杆菌中表达。结果克隆了中国人肥胖基因，其ｃＤＮＡ顺序与已报道的白种人的序列完全一致，并成功在大肠杆菌中获得表达。结论本项工作为进一步研究肥胖基因在肥胖症和糖尿病等病人中的表达情况及探讨肥胖基因的作用机制打下了基础。Objective To probe into the positional cloning of the Chinese obese gene, thereby providing an important approach to research of the mechanisms of obesity. Methods The obese gene was amplified from the Chinese adipose tissue RNA by RT PCR. Results Sequencing analysis showed that the Chinese obese gene cDNA sequence was identical to that of Caucasian reported previously. The gene was cloned into a prokaryotic expression vector and high efficiency expression was realized in E.coli. Conclusion This work is the basis for further researches on mechanisms of the obese gene product leptin and its potential effect on treatment of obesity and diabetes mellitus, etc.

关 键 词：肥胖基因 分子克隆 肥胖症 大肠杆菌 

分 类 号：R589.202[医药卫生—内分泌] R378.21[医药卫生—内科学]