热休克蛋白70-hom基因+2437(T/C)位点基因多态性与肺癌的关系  

Heat shock protein 70-hom gene+2437(T/C) polymorphism and susceptibility to lung cancer in Chinese Han population

作　　者：王玉珍[1] 吴逸明[1] 李伟辉[1] 梁守沛[1] 周舫[1] 

机构地区：[1]郑州大学公共卫生学院劳动卫生学教研室,郑州450001

出　　处：《郑州大学学报（医学版）》2010年第2期219-222,共4页Journal of Zhengzhou University(Medical Sciences)

基　　金：国家自然科学基金资助项目30600490;国家自然科学基金资助项目30571552;河南省教育厅科技创新人才支持计划2008HASTIT028;河南省教育厅自然科学研究资助项目2008A320018

摘　　要：目的：研究热休克蛋白（HSP）70-hom基因＋2437（T／C）单核苷酸多态性与肺癌遗传易感性的关系，探讨肺癌易感的HSP70一hom基因型。方法：应用聚合酶链反应-限制性片段长度多态性（PCR—RFLP）方法检测159例肺癌患者和202名正常对照人群HSP70-hom基因＋2437（T／C）位点多态性，采用病例一对照研究分析其与肺癌的关系。结果：病例组与对照组之间HSP70．hom各基因型（TT、TC和CC）频率及T和c的等位基因频率分布，差异均有统计学意义（P〈0．05）。对病理类型分层分析并经年龄、性别、吸烟状况、饮酒史和肿瘤家族史调整后发现，非TT基因型携带者患肺鳞癌危险性是TT基因型携带者的2．17倍（95％CI：1．17～4．00）。对吸烟状况进行分层分析并经年龄、性别、饮酒史、肿瘤家族史调整后发现，吸烟者及重度吸烟者中非TT基因型调整OR值分别为5．19（95％CI：1．57～17．19）和9．23（95％CI：2．52～33．84），与肺癌有强关联，提示TT基因型是吸烟者特别是重度吸烟者的保护因素。结论：HSP70一hom基因＋2437（T／C）位点TT基因型可能是肺鳞癌的遗传保护因素，并可降低吸烟者特别是重度吸烟者患肺癌的危险度。Aim： To investigate the correlation between the heat shock protein 70-hom gene polymorphism and lung cancer. Methods：A case-control study of 159 lung cancer patients and 202 control subjects was carried out to investigate the relationship between the polymorphisms of HSP70-hom gene ＋ 2 437 site and lung cancer. The genotypc frequencies was identified by polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP）. Results：Significant differ- ences were found in the distribution of HSP70-hom genotypes and allele frequencies between lung cancer patients and con- trols（ P 〈 O. 05 ）. After stratified analysis of pathological types and adjusting by age, gender, smoking history, drinking sta- tus and family history, it was revealed that non-TT genotype was associated with increased risk of lung squamous cell carci- noma, OR = 2.17 （95% CI： 1.17 -4. O0 ）. After stratified analysis of smoking history and adjusting by age, gender, drink- ing status and family history, it was revealed that non-TT genotype was associated with increased risk of lung cancer in smokers group and heavy-smokers group, OR = 5.19 （ 95 % CI： 1.57 ~ 17.19 ） and 9.23 （ 95 % CI： 2.52 - 33.84 ）. Conclu- sion： HSPT0-hom ＋2 437（T/C） TT genotype may act as a protective factor in lung squamous cell carcinoma patients and may decrease the risk of lung cancer in smokers group, especially in heavy-smokers group.

关 键 词：热休克蛋白70-hom基因多态性 肺癌 

分 类 号：R734.2[医药卫生—肿瘤]