河南汉族人群同胞兄弟短串联重复序列多态性测定  

Detection of polymorphism of short tandem repeat in male sibling of Henan Han population

作　　者：李杨[1] 孙莉[2] 李晓文[1] 陈辉[1] 

机构地区：[1]郑州大学基础医学院细胞生物学与医学遗传学教研室,郑州450001 [2]郑州大学第一附属医院检验科,郑州450052

出　　处：《郑州大学学报（医学版）》2010年第2期278-281,共4页Journal of Zhengzhou University(Medical Sciences)

摘　　要：目的:探讨Y染色体短串联重复序列(Y-STR)位点和常染色体STR位点联合用于鉴定同胞兄弟关系的可行性。方法:PCR复合扩增河南汉族80对同胞(包括34对兄弟)和80对无关个体(包括51对男性无关个体)的7个Y-STR位点(DYS19、DYS385、DYS389Ⅰ、DYS389Ⅱ、DYS390、A7.1和H4)和13个常染色体STR位点(CSFIPO、TPOX、THOI、F13AOI、VWA、D16S539、D7S820、D13S317、D18S51、D3S1358、D21S11、D8S1179和D5S818),比较同胞组和无关个体组等位基因的匹配情况。结果:不同家系的同胞对和无关个体有相同单倍型出现。选取13个常用的常染色体STR位点检测,同胞组和无关个体组分别有5对和15对不能判断。联合运用这20个位点,仅有5对同胞不能确定。结论:Y-STR位点与常染色体STR位点联合用于同胞兄弟鉴定可以大大提高准确率。Aim： To explore the feasibility in identifying male siblings by Y-short tandem repeat（STR） and autosomal STR. Methods： Both 7 Y-STR （ DYS19, DYS385, DYS389 I , DYS389 Ⅱ , DYS390, A7.1 , and H4 ） and 13 autosmal STR （ CSFIPO ,TPOX, THOI, F13AOI, VWA, D16S539, D7S820, D13S317, D18S51, D3S1358, D21 S11, D8S1179, and D5 $818 ） were amplified by multiple PCR to study 80 pairs of siblings and 80 pairs of unrelated individuals. Then allelic matching of two groups were compared. Results ：The same haplotype was shown in different pairs of siblings and the unrelated individu- als. 5 pairs in the group of siblings and 15 pairs in the groups of unrelated individuals could not be identified by analyzing 13 autosomal STR loci ,while only 5 pairs in the group of siblings could not be judged by combining 7 Y-STR and 13 autoso- mal STR. Conclusion ： It is more effective that Y-STR and automal STR are jointly used to identify male siblings.

关 键 词：短串联重复序列 同胞兄弟 等位基因 河南省 汉族 

分 类 号：Q347[生物学—遗传学]