肾小球疾病儿童体内游离氨基酸含量的研究  

Free Animo Acids Level in the Plasma of Children suffering from Glomerular Diseases

作　　者：曹斌[1,2] 程江[1,2] 古桂雄[1,2] 蒋莹[1,2] 黄美英[1,2] 

机构地区：[1]苏州医学院附属儿院 [2]苏州医学院生化教研室

出　　处：《氨基酸和生物资源》1998年第4期10-13,共4页Amino Acids & Biotic Resources

摘　　要：为了研究小儿肾小球病变时体内游离氨基酸的代谢变化，采用丹酰氯聚酰胺薄层分析法研究了原发性肾病综合症（ＩＮＳ）８例、急性肾小球肾炎（ＡＧＮ）１２例、过敏性紫癜肾炎（ＡＰＮ）７例与正常对照组２９例的血清及红细胞内游离氨基酸含量的变化。结果表明：（１）此三种肾小球疾病，血清中苯丙氨酸（Ｐｈｅ）、脯氨酸（Ｐｒｏ）、色氨酸（Ｔａｐ）、赖氨酸（Ｌｙｓ）、甘氨酸（Ｇｌｙ）明显高于正常，导致酪／苯丙（Ｔｙｒ／Ｐｈｅ）、缬／甘（Ｖａｌ／Ｇｌｙ）等分子比降低，提示肾功能受损。（２）血清支链氨基酸（ＢＣＡＡ）的含量在ＩＮＳ中低于正常组（ｔ＝３．４８；Ｐ＜０．０１），而在ＡＧＮ、ＡＰＮ中却高于正常组（ｔ分别为２．３３，２．３９，Ｐ＜０．０５）。（３）红细胞中丝氨酸（Ｓｒｅ）、苯丙氨酸（Ｐｈｅ）、色氨酸（Ｔｒｐ）、胱氨酸＋半胱氨酸（Ｃｙｓ）、天冬氨酸（Ａｓｐ）、谷氨酸（Ｇｌｕ）的含量ＡＰＮ组高于ＩＮＳ及ＡＧＮ，提示ＡＰＮ患儿中肾功能损害没有ＩＮＳ及ＡＧＮ严重。The FAA level in plasma and Red Blood Cell (RBC) from 8 children suffering from idiopathic nephrotic syndrome (INS), 12 suffering from acute glomerulonephritis (AGN), 7 suffering from allergic purpure nephrits (APN) and 29 normal group were determined with Densly cholrids reaction and thin layer chromatography (TLC). The results showed that (1) the level of phenylalanine (Phe), proline(Pro), tryptophan(Tyr), lysine(Lys) and glycine(Gly) in the plasma of the patients suffering from the three kinds of glomerular diseases were significantly higher than those of normal grope, which decreased the ratio of Tyr/Phe and Val/Gly, and indicated that the renal function has been damaged; (2)the level of branched chain acids (BCAA) in the plasma from INS was lower than that from normal (t=3.48;P<0.01), amino but was higher the plasma from AGN and INS than that from normal (t=2.33,2.39 respecterly, P<0.05), and (3) the content of serine (Ser), Phe, Try, cysteine (Cys), aspartic acid(Asp), glutamic acid(Glu) in RBC from APN was highter than those from INS and from AGN, which indicated that the damage of renal function in APN was Less serious than that in INS and in AGN.

关 键 词：游离氨基酸 血清 红细胞 肾小球疾病 儿童 

分 类 号：R692.6[医药卫生—泌尿科学]