Determination of Protein via Resonance Light Scattering Technique with (NH_4)_2SO_4  

作　　者：WU Li-hang MU Dan LIANG Fang-hui TIAN Yuan ZHANG Han-qi YU Ai-min WANG Xing-hua CHEN Ying 

机构地区：[1]College of Chemistry,Jilin University,Changchun 130012,P.R.China [2]Department of Pharmaceutical Science,Changchun Medical College,Changchun 130031,P.R.China [3]Jilin Province Center of Environmental Monitoring,Changchun 130011,P.R.China

出　　处：《Chemical Research in Chinese Universities》2010年第2期189-193,共5页高等学校化学研究（英文版）

摘　　要：Be able to form large particles with protein via electrostatic force to enhance the intensity of resonance light scattering（RLS）,（NH4）2SO4 was used as a RLS probe to determine bovine serum albumin（BSA）,human serum albumin（HSA） and ovum albumin（OVA）.The experimental conditions were investigated,including the acidity and saturation degree of （NH4）2SO4.Under the optimum conditions,the enhanced RLS intensity is proportional to the concentration of BSA,HSA and OVA in the ranges of 5×10-8―1×10-6,2.5×10-8―8×10-7 and 5×10-8―1.5×10-6 mol/L,respectively.The detection limits for BSA,HAS and OVA are 6.6×10-9,3.8×10-9 and 7.4×10-9 mol/L,respectively.The effects of foreign substances were also examined.The practical and synthetic samples were analyzed with satisfactory results.Be able to form large particles with protein via electrostatic force to enhance the intensity of resonance light scattering（RLS）,（NH4）2SO4 was used as a RLS probe to determine bovine serum albumin（BSA）,human serum albumin（HSA） and ovum albumin（OVA）.The experimental conditions were investigated,including the acidity and saturation degree of （NH4）2SO4.Under the optimum conditions,the enhanced RLS intensity is proportional to the concentration of BSA,HSA and OVA in the ranges of 5×10-8―1×10-6,2.5×10-8―8×10-7 and 5×10-8―1.5×10-6 mol/L,respectively.The detection limits for BSA,HAS and OVA are 6.6×10-9,3.8×10-9 and 7.4×10-9 mol/L,respectively.The effects of foreign substances were also examined.The practical and synthetic samples were analyzed with satisfactory results.

关 键 词：Resonance light scattering （NH4）2SO4 Bovine serum alumin Human serum alumin Ovum albumin 

分 类 号：Q512.1[生物学—生物化学] TQ925.9[轻工技术与工程—发酵工程]