胃腺癌细胞株和高转移株中雌激素受体相关受体的表达  

Expressions of Estrogen Receptor Related Receptors in Gastric Adenocarcinoma Cell Lines and Hypsi-invasion Cell Lines

作　　者：唐成和[1] 杨留才[1] 钱靖宇[1] 

机构地区：[1]盐城卫生职业技术学院,江苏盐城224006

出　　处：《苏州大学学报（医学版）》2009年第6期1069-1071,共3页Suzhou University Journal of Medical Science

基　　金：盐城市医学科技发展计划项目(2008121)

摘　　要：目的探讨雌激素受体相关受体(ERR)α、β、γ各亚型在胃腺癌细胞系SGC-7901及高转移株OCUM-2MD3中的表达情况。方法采用RT-PCR法定性检测ERRs家族各亚型的mRNA表达,采用Western blot蛋白印迹检测ERR各亚型的表达。结果ERRα在SGC-7901低表达、在OCUM-2MD3高表达;ERRβ基本无变化;ERRγ在SGC-7901高表达、在OCUM-2MD3低表达。结论在不同类型的胃腺癌细胞株中,ERRα和ERRγ表达方式不同,提示用ERRα/ERRγ比值的改变显示胃腺癌转移有一定的临床意义。Objective To explore the expression of estrogen receptor related receptor （ERR） α, β and γ in gastric adenoearcinoma cell lines SGC-7901 and hypsi-invasion cell lines OCUM-2MD3. Methods RT-PCR was used to detect the mRNA expression and Western blot was used to be detected the protein expression of ERRs. Results ERR α was detected with low expression levels in SGC-7901, but with was high expression levels in OCUM-2MD3. ERRβ was detected with no change in SGC-7901 and OCUM-2MD3. While ERRs/was detected with high expression levels in SGC-7901, but with low expression levels in OCUM-2MD3. Conclusion ERRs and ERRs/were detected in gastric adenocareinoma cell lines. This study provides a in vitro expression pattern of cancer cell lines for ERRs family, the ratio change of ERRα/ERR-γ may play a role in the metastasis of gastric cancer.

关 键 词：胃癌 雌激素受体相关受体 转移力 内分泌治疗 

分 类 号：R735.2[医药卫生—肿瘤]