基质金属蛋白酶抑制剂在滤过性手术中抗瘢痕作用的实验研究  

EXPERIMENTAL STUDY OF ANTIPROLIFERATIVE EFFECTS OF MMPS INHIBITORS DURING FILTERING OPERATION

作　　者：申颖[1] 李斌[2] 

机构地区：[1]内蒙古医学院附属医院眼科,内蒙古呼和浩特010050 [2]内蒙古医学院

出　　处：《内蒙古医学院学报》2010年第1期13-17,共5页Acta Academiae Medicinae Neimongol

基　　金：内蒙古医学院青年基金(NY2009QN006)

摘　　要：目的:研究基质金属蛋白酶抑制剂GM6001对兔眼滤过性术后抗瘢痕作用。方法:取青紫蓝兔24只,双眼行小梁切除术。术后右眼结膜下注射GM6001,左眼注射PBS作为对照,术后测量眼压,观察结膜、角膜、前房、晶状体情况,记录功能性滤过泡数,并进行HE染色,光镜观察。结果:术后眼压较术前下降,术后各时间点实验组眼压均较对照组眼压低,且有显著性差异(P<0.01)。术后未出现角膜水肿、晶状体混浊、浅前房等并发症,轻微的结膜充血和前房反应于术后7d基本消失且两组无明显差别。实验组较对照组功能性滤过泡形成率高。结论:GM6001具有抑制兔青光眼滤过术后滤过道中成纤维细胞过分增生,减少瘢痕形成的作用,延长了功能性滤过泡存活的时间。Objective：To study the antiproliferative effects of MMPS （matrix metalloproteinases, MMPS） inhibitors GM6001 during filering operation on rabbit eyes. Methods ：Twenty -four pigmented rabbits were employed auto -control. All rabbits （48 eyes） underwent trabeculectomy. The eyes （OD） were injected subconjunctivally with GM6001 in experiment group and the eyes（OS） were in- jected subconjunctivally with PBS after surgery in control group. The intraocular pressure （IOP） ,filtering bleb and inflammatory reation were observed. Results：Intraocular pressure （IOP） was significantly lower in experiment group （GM6001） than in the control group after surgery （P 〈 0, 01 ）. Light conjunctival congestion and inflammatory in anterior chamber had disappeared 7 days after surgery and haven＇t the corneal edema and other complications. The number of functioning blebs without connective tissue collagenization were showed statistically significant differences between the control and experiment group after the surgery. Conclusion： GM6001 have the efficency of inhibit the fibroblast ceils pro- liferation in filtering pathway, show better IOP control and bleb survival in this experimental model of filtration operation.

关 键 词：基质金属蛋白酶抑制剂 GM6001 滤过术 瘢痕 

分 类 号：R775.2[医药卫生—眼科]