绿茶及其提取物抗耐甲氧西林金黄色葡萄球菌作用研究  

Study of antibacterial activity of green tea and its extracts against MRSA

作　　者：华德兴[1,2] 彭青[1] 曾香连[2] 姚芬[1] 钱元恕[1] 

机构地区：[1]汕头大学医学院药理教研室,汕头515041 [2]汕头大学医学院附属粤北人民医院,韶关512026

出　　处：《中国抗生素杂志》2010年第3期228-233,共6页Chinese Journal of Antibiotics

摘　　要：目的研究绿茶及提取物对耐甲氧西林金黄色葡萄球菌(MRSA)的抗菌作用并分析其主要抗菌成分。方法采用琼脂稀释法测定各种茶叶的水浸出物及茶叶的主要成分对MRSA的MIC。纸片扩散法观察绿茶浸出物和茶叶主要成份与抗生素合用对MRSA的协同抗菌情况。结果①绿茶浸出物对MRSA的MIC范围为0.125-0.5%,青茶浸出物为0.125-0.5%,白茶浸出物为0.0625-0.25%,红茶浸出物为0.5%,黑茶浸出物为0.25-0.5%。②茶多酚对MRSA的MIC范围为128-256μg/mL,茶色素为256-512μg/mL,茶多糖为512-1024μg/mL,茶皂素和咖啡因则均大于1024μg/mL。③青霉素、苯唑西林、氨苄西林、头孢他啶、头孢替唑、米诺环素、四环素与绿茶浸出物合用的抑菌圈明显大于单用抗生素的抑菌圈,红霉素和氯霉素单用/合用的抑菌圈相同,环丙沙星加入绿茶浸出物后抑菌圈反而减小。④茶多糖、茶皂素、茶色素、咖啡因与苯唑西林单用/合用的抑菌圈均相同,茶多酚与苯唑西林合用的抑菌圈明显大于单用苯唑西林的抑菌圈,表儿茶素没食子酸酯(ECg)/表没食子儿茶素没食子酸酯(EGCg)与苯唑西林合用的抑菌圈明显大于各自单用的抑菌圈。结论各种茶叶对MRSA均有一定的抗菌作用,其中绿茶和白茶的抗菌作用最强。绿茶抗MRSA的主要成分为茶多酚,并且茶多酚对β-内酰胺类抗生素和四环素抗MRSA有增效作用,但对其它类抗生素呈无关或拮抗作用。茶多酚中的2个单体ECg和EGCg能增强苯唑西林的抗MRSA作用。Objective To study on antibacterial activity of green tea and its activity against MRSA, and to analyze its main components against MRSA. Methods The MICs of several kinds of tea and the main components of tea against MRSA were performed by agar dilution method. Synergetic antibacterial test of green tea leachate or tea main components with antibiotics against MRSA were carried out by Kirby-Bauer. Results ①The MIC range of green tea leachate was from 0.125% to 0.5%, oolong tea was from 0.125% to 0.5%, white tea was from 0.0625% to 0.25%, dark tea was from 0.25% to 0.5% and black tea was 0.5%. ② The MIC values of tea polyphenol were 128- 256μg/mL, values of tea Pigment were 256- 512μg/mL, values of tea polysaccharide were 512-1024μg/mL, and values of tea saponin and caffein were exceeded 1,024μg/mL. ③Inhibition zone of penicillin, oxacillin, ampicillin, ceftazedime, ceftezole, minocycline and tetracycline in combination with green tea leachate against MRSA were obviously larger than the single antibiotics. Inhibition zone of erythrocin and chloromycetin against MRSA were not increased when added green tea leachate. While inhibition zone of ciprofloxacin against MRSA were decreased after adding green tea leachate. Inhibition zone of tea polyphenol in combination with Oxaeillin against MRSA were obviously larger than the single drug, and ECg/EGCg in combination with Oxacillin against MRSA were also obviously larger than the single drug. Conclusion All kinds of tea show anti-MRSA activity, and green tea and white tea show strongest anti-MRSA activity. The main component against MRSA in green tea is tea polyphenol, and tea polyphenol could enhance the anti-MRSA activity of β-1actam antibiotics and Tetracycline. Two monomers of tea polyphenol, ECg and EGCg, show the ability to enhance the anti-MRSA activity of oxacillin.

关 键 词：耐甲氧西林金黄色葡萄球菌 茶 绿茶 抗菌 

分 类 号：Q946.887[生物学—植物学]