荧光原位杂交及荧光定量PCR技术检测急性早幼粒细胞白血病患者PML-RARα融合基因比较  

Comparison between FISH and fluorescent quantitative PCR in detecting PML-RARα fusion gene of APL patients

作　　者：李蔚[1] 李艳丽[1] 赵雪飞[1] 邱林[1] 马军[1] 

机构地区：[1]哈尔滨血液病肿瘤研究所,150010

出　　处：《白血病．淋巴瘤》2010年第3期143-145,共3页Journal of Leukemia & Lymphoma

摘　　要：目的 比较荧光原位杂交（FISH）及荧光定量PCR技术对急性早幼粒细胞白血病（APL）患者PML-RARα融合基因检测的灵敏度和特异度.方法 选取75例APL患者,同时应用FISH及荧光定量PCR方法检测患者PML-RARα融合基因的表达情况.结果 共检测初发与复发或缓解患者88例次,总相符率为96.59%;检测初发患者14例,相符率为100%;检测复发或缓解患者74例次,相符率为95.95%;检测干细胞来源的标本6例次,相符率为100%.结论 对于初发的APL患者,FISH与荧光定量PCR的灵敏度相同,FISH技术操作更为简单,省时直观.对于残留的检测,FISH的灵敏度不如荧光定量PCR.Objective To detect the PML-RARα fusion gene expression of APL patients with FISH and fluorescent quantitative PCR and discuss the sensitivity and specificity of two techniques. Methods The detection of the PML-RARα fusion gene expression of 75 APL patients with FISH and fluorescent quantitative PCR were carried out simultaneously. Results Eighty eight patients of primary and relapse or remission phases were examinated and total conformity rate was 96.59 %. Fourteen primary patients were detected and conformity rate was 100 %. Seventy four relapse or remission patients were detected and conformity rate was 95.95 %. Stem cell essays were detected for six times and conformity rate was 100 %. Conclusion The sensitivity of FISH and fluorescent quantitative PCR is identical for primary APL patients and FISH is more sensitive. But the sensitivity of FISH is weaker than that of fluorescent quantitative PCR for detection of residue disease.

关 键 词：原位杂交 荧光 聚合酶链反应 基因融合 

分 类 号：R733.7[医药卫生—肿瘤]