3q27-3q29相关的p63蛋白表达在弥漫性大B细胞淋巴瘤中的意义  

Significance of 3q27-3q29-related p63 protein expression in diffuse large B-cell lymphoma

作　　者：陈振文[1] 王晋芬[2] 王国平[4] 张宏伟[3] 刘冬梅[1] 徐义荣[1] 张朝霞[1] 田林[5] 

机构地区：[1]山西医科大学汾阳学院病理学教研室,032200 [2]山西省肿瘤医院病理科 [3]山西省肿瘤医院血液科 [4]华中科技大学同济医学院病理学系 [5]山西医科大学附属汾阳医院病理科

出　　处：《白血病．淋巴瘤》2010年第3期161-165,共5页Journal of Leukemia & Lymphoma

基　　金：山西省青年科技研究基金项目（2007021055）

摘　　要：目的 探讨3q27-3q29相关的p63蛋白表达在弥漫性大B细胞淋巴瘤（DLBCL）中的意义.方法 应用免疫组织化学EnVision二步法检测102例DLBCL与15例反应性淋巴组织增生（RHL）中p53蛋白和3q27-3q29相关的p63蛋白的表达并进行随访.结果 p53、p63蛋白在DLBCL中的阳性率分别为62%和56%,在RHL中的阳性率分别为0和13%,p53、p63蛋白在DLBCL与RHL中比较差异均有统计学意义（P〈0.05）;p53、p63蛋白的表达在Ⅰ＋Ⅱ期中的阳性率分别为48.3%和41.4%,在Ⅲ＋Ⅳ期中的阳性率分别为79.5%和75%,差异有统计学意义（P〈0.05）;p53、p63蛋白的表达在生发中心B细胞型（GCB型）中的阳性率分别为28%和28%,在非GCB型（non-GCB型）中的阳性率分别为72.7%和64.9%,差异均有统计学意义（P〈0.05）.p53、063蛋白表达与患者性别、年龄、有无B症状和发病部位等无相关性（P〉0.05）.p53蛋白表达与p63蛋白表达呈正相关性（r=0.629,P〈0.05）.p53、063蛋白表达阴性组的5年总体生存率高于阳性组（38%：6%,51%：4%）,差异有统计学意义（P〈0.05）.结论 p63可能作为癌基因与p53共同参与了DLBCL的发生和发展,两者在肿瘤的发生中可能起着协同作用;联合检测p63蛋白和p53蛋白在DLBCL中的表达,可能成为判断DLBCL预后指标之一.Objective To investigate the expressions and clinicopathological significance of 3q27-3q29-related p63 protein in diffuse large B-cell lymphoma （DLBCL）. Methods An immunohistochemical Envision^（TM） method was used to detect the expressions of p53 and 3q27-3q29-related p63 protein in 102 cases of DLBCL and 15 cases of reactive hyperplasia of lymph node （RHL）. Results The tumor cell expressions of p53（62 %） and p63（56 %） in DLBCL were significantly higher than that in RHL （0 and 13 % P 〈 0.05）. The expressions of p53 and p63 were significantly different （1） between stage Ⅰ ＋ Ⅱ （the positive rate 48.3 % and 41.4 %, respectively） and stage Ⅲ＋Ⅳ（the positive rate 79.5 % and 75 %, respectively; P 〈0.05）, （2） between GCB type （the positive rate 28 % and 28 %, respectively） and non-GCB type（the positive rate 72.7 % and 64.9 %, respectively; P 〈0.05）. The expressions of p53 and p63 had no relationship to gender, age, B symptoms and locations. The expression of p53 was positively correlated with that of p63 in DLBCL （P 〈0.05, Cp=0.629）. p53 and p63 protein expression in negative group the 5-year overall survival rate is higher than that in positive group （38 % and 6 %, 51% and 4 %, respectively）, the difference was statistically significant （P 〈0.05）. Conclusion It was likely that p63, as the oncogene, participated in the occurrence and development of DLBCL together with p53. Also, p63 and p53 might play a synergistic role in the occurrence DLBCL. Combined detection of 3q27-3q29-related 1963 protein and p53 protein in DLBCL, might be one of the indicators to the prognosis of DLBCL.

关 键 词：淋巴瘤 大细胞 弥漫型 基因 P53 P63 3q27-3q29 免疫组织化学 

分 类 号：R733.1[医药卫生—肿瘤]