乳腺癌患者T细胞受体基因重排及CDR3区序列分析  

Analysis of the TCR β-chain gene rearrangement and CDR3 repertoire in patients with breast cancer

作　　者：张建波[1] 宋永平[2] 于庆凯[1] 胡俊[3] 董涛[3] 

机构地区：[1]河南省肿瘤医院病理科,郑州450003 [2]河南省肿瘤医院血液科,郑州450003 [3]中山大学中山医学院微生物学教研室

出　　处：《肿瘤研究与临床》2010年第3期179-181,共3页Cancer Research and Clinic

摘　　要：目的分析乳腺癌转移淋巴结穿刺标本中T细胞受体（TCR）B链克隆性基因重排及互补决定区3（CDR3）区序列。方法应用RT—PCR扩增2例反应性增生淋巴结及3例乳腺癌转移淋巴结T细胞24个TCR Vβ亚家族的CDR3基因，并经基因扫描确定T细胞克隆性，对提示单克隆或寡克隆增生亚家族的PCR产物进行测序。结果乳腺癌转移淋巴结T细胞中TCR存在限制性取用，仅表达3～5个Vβ亚家族。增生的T细胞存在克隆性特点，增生形式包括寡克隆及多克隆。CDR3区序列分析证实增生的T细胞克隆具有不同的氨基酸序列。结论乳腺癌转移淋巴结中存在T细胞克隆性增生，其TCRVβ呈限制性取用，不同T细胞克隆的CDR3序列不同。Objective To analyze the clonal gene rearrangement and complementarity determining region 3 （CDR3） repertoire of TCR β-chain in fine needle aspiration biopsy （FNAB） specimens of lymph node metastasis in patients with breast cancer. Methods The TCR CDR3 region ,genes of 24 TCR VI3 subfamilies were amplified by utilizing RT-PCR technology, and the CDR3 lengths of TCR β-chain were analyzed with gene scan technology for 2 cases with lymph node reactive hyperplasia and 3 patients with lymph node metastasis of breast cancer. The clonality of T cells presumed by spectra typing was further confirmed by CDR3 sequencing. Results TCR 13-chain presented specific repertoire skewing in metastatic lymph node, and only 3-5 TCR Vβ subfamily of T cells were identified, respectively. Clonal expanded T cells, including oligoclonal, polyclonal patterns, in one or more Vβ subfamilies were found in all cases. The oligoclonal expanded T cells had different CDR3 amino acid sequences. Conclusion There are characteristic T cells cloning proliferation and selected usage of TCR Vβ subfamily T cells could be found in metastatic lymph node.The sequences of CDR3 in different TCR clone proliferation are mostly different.

关 键 词：乳腺肿瘤 基因重排 互补决定区 基因扫描 

分 类 号：R737.9[医药卫生—肿瘤]