产胞外木聚糖酶放线菌的分离与筛选  

SCREENING OF EXTRACELLULAR-XYLANASE-PRODUCING Actinomyces

作　　者：孙迅[1] 朱陶[1] 朱启忠[1] 孙宝聚 

机构地区：[1]山东省菏泽师范专科学校生物系,274015

出　　处：《微生物学杂志》1998年第4期29-32,共4页Journal of Microbiology

摘　　要：报道了产胞外木聚糖酶放线菌的筛选方法。实验表明，PH6.0±0．5可用作一般放线菌木聚糖酶活力测定的酸碱度。分离并筛选了一株产胞外木聚糖酶活力高的链霉菌（Streptomycessp．Strz－6），酶活力达9．77IU／ml，酶最适作用温度和酸碱度分别为55℃和pH6．5；Na+、K+、Ca2+等离子对木聚糖酶有激活作用，而Fe3+、Zn2+、Cu2+等离子明显抑制酶的活性。This paper reports the method for screening extracellular - xylanase - producing Actinomyces. The results showed the pH 6.0±0. 5 can generally be used for acidity and/or alkalinity detedrination of the xylanase activity of Actinomyces. Streptompees sp. Str - z - 6 that preduced high yield of extracellular xylanase was screened. The enzyme activity was9. 77IU/ml. The optimal telnperature and pH for xylanas activity were 55℃ and pH 6. 5 respectively. Na+, K+ and Ca2+ increased the enzyme activity, whereas Fe3+, Cu2+ and Ag+ obviously inhibited it.

关 键 词：木聚糖酶 放线菌 筛选 酶法制浆 

分 类 号：TS743.14[轻工技术与工程—制浆造纸工程] Q939.13[生物学—微生物学]