用自身次生代谢产物抗性筛选宁南霉素高产菌株  

SELECTION OF NINGNANMYCIN OVERPRODUCTION STRAINS BY RESISTANCE OF SELF-SECONDARY METABOLITES

作　　者：陈家任[1] 陈弘[2] 胡厚芝[1] 

机构地区：[1]中国科学院成都生物研究所,成都610041 [2]重庆建筑大学

出　　处：《微生物学报》1998年第6期484-486,共3页Acta Microbiologica Sinica

基　　金：国家"九.五"科技攻关项目部分内容.

摘　　要：宁南霉素是诺尔斯链霉菌西昌变种产生的一种胞嘧啶核苷肽型新抗生素,它对多种病毒、细菌和真菌引起的作物病害都有较好的防治效果,毒性低、残留量小,无蓄积作用,具有良好的应用前景.然而它的原始菌株 16A—6发酵单位仅 700U/ml^1000U/ml,严重阻碍了它的推广应用.The S. noursei var. xichangensis FR-5 protoplasted was treated by UV, NTG sequencely, and the mutated strains were selected by use of self-secondary metabolites, the microorganisms were isolated by fermentation after agarblock rational method. The seven strains of microorganisms which were one fold production higher than that of ogiginal strain were obtained from 122 mutants. The method should be a simple, high speed selection model of breed Ningnanmycin overproduction strains of microorganisms.

关 键 词：宁南霉素 抗性筛选 高产菌株 次生代谢产物 

分 类 号：TQ927[轻工技术与工程—发酵工程] TQ920.1