17种植物中蛋白质提取物的抗HIV-1活性  

Anti Human Immunodeficiency Virus Activity of Proteins from 17 Species of Plants

作　　者：郑永唐[1,2] 贲昆龙[1,2] 金善炜[1,2] 

机构地区：[1]中国科学院昆明动物研究所 [2]中国科学院上海有机化学研究所

出　　处：《中国病毒学》1998年第4期312-321,共10页Virologica Sinica

基　　金：中国科学院"九.五"重大应用项目;"西部之光"人才基金;云南省应用基础研究基金;云南省中青年学术和技术带头人后备人才基金

摘　　要：以化合物对ＨＩＶ１诱导Ｃ８１６６细胞形成合胞体的抑制实验和化合物对ＨＩＶ１感染细胞的保护实验作为初筛方法，筛选了来源于７科１７种植物的４７个样品，其中８个样品经测定是核糖体失活蛋白（ＲＩＰｓ），其余为粗提蛋白。进而测定了初筛有抗ＨＩＶ活性的化合物对共培养、急性和慢性感染的ＨＩＶ１ｐ２４抗原表达水平的影响，用间接荧光染色检测这些化合物对ＨＩＶ抗原阳性细胞率的影响，以确证其抗ＨＩＶ活性。天花粉蛋白（ＴＣＳ）、南方栝楼蛋白峰Ⅴ、南方栝楼蛋白峰Ⅵ均显著地抑制合胞体的形成；巴Ⅱ、丝瓜子蛋白、油瓜根蛋白、木盍藤子蛋白有一定的抑制作用。老鼠拖瓜蛋白、大叶木鳖子根蛋白、西双版纳根Ⅱ等粗提蛋白有显著的抑制作用。ＲＩＰｓ不能保护ＨＩＶ１感染细胞的死亡。ＴＣＳ显著地抑制了ＨＩＶ１急性感染中ｐ２４抗原的表达，减少了ＨＩＶ抗原阳性细胞数，但它们均不影响共培养细胞融合、ＨＩＶ１慢性感染中ｐ２４抗原表达水平。结果表明，除ＴＣＳ等已知ＲＩＰｓ外，还有一些新的ＲＩＰｓ具有不同程度的抗ＨＩＶ活性。In order to find new anti human immunodeficiency virus (HIV) ribosome inactivating proteins (RIPs), the anti HIV activities of 47 RIPs and crude proteins from 17 species of plants were examined by the following measurements: 1) the inhibition of syncytia formation; 2) the protection of HIV 1 infected cell;3) inhibition of HIV 1 infected cell fusion in coculture;4) reduction of p24 antigen expression and numbers of HIV antigen positive cells in acutely and chronically infected culture. Among 47 proteins, trichosanthin (TCS) (SI=193.3) from Trichosanthes kirilowii, protein fractions Ⅴ (SI=243) and Ⅵ (SI>1200) from Trichosanthes damiaoshanesis were found to obviously inhibit syncytia formation induced by HIV 1. Crotin Ⅰ from Croton tiglium, luffin from Luffa cylinarica , RIPs from Hodgsonia macrocarpa and Entada phaseoloides showed slightly inhibition of the syncytia formation (SI<50). Crude protein extracts from Trichosanthes Ovigera, Momordica macrophylla and an unnamed plant growing in Xishuangbanna district, Yunnan province, obviously inhibited syncytia formation, but proteins purified from them lost their activities. All RIPs were unable to block HIV infected cell fusion in coculture, and did not protect HIV 1 infected MT 4 or C8166 cells from dying. TCS markedly reduced both expression of p24 core protein and the numbers of HIV antigen positive cells in acutely but not chronically HIV 1 infected culture. These data suggested that in addition to TCS and the known RIPs, some of the other RIPs may have anti HIV activity to certain extent.

关 键 词：人免疫缺陷病毒 抗病毒活性 植物蛋白质 

分 类 号：R282.710.5[医药卫生—中药学] R512.91[医药卫生—中医学]