AC-PCR法检测连云港海域贝类HAV  

Detection of HAV Contamination in Shellfish from Lianyungang Sea Water by Antibody Capture PCR

作　　者：赵文彬[1,2] 林红玉[1,2] 李家富 王理兴[1,2] 龚海萍 方肇寅 王秋红[1,2] 

机构地区：[1]连云港市卫生防疫站 [2]中国预防医学科学院病毒学研究所

出　　处：《中国病毒学》1998年第4期369-372,共4页Virologica Sinica

摘　　要：为了解连云港海域贝类甲型肝炎病毒（ＨＡＶ）污染状况，证实其在本地区甲肝传播中的媒介地位，我们应用抗体捕捉聚合酶链反应（ＡＣ／ＰＣＲ）检测市售贝类的ＨＡＶ，结果报告如下：材料和方法１贝类样品于１９９６年春、秋二季采集新浦区、连云区和赣榆县等地水产品市场...A method of antibody capture polymerase chain reaction (AC PCR) is developed for the detection of human hepatitis A virus (HAV) in shellfish ( Scapharca subcrenata, Buditapes philippinarum, Macoma incongrua, Sinonovacula constricta, Chlamys farrer ). Initial virus extraction is based on a modified elution precipitation technique. Eleven out of 31 shellfish samples from Lianyungang (in Jiangsu Province) sea water are HAV positive. This experiment proves that the consumption of shellfish growing in contamination waters is associated with the risk of transmission of HAV.

关 键 词：贝类 甲型肝炎病毒 聚合酶链反应 

分 类 号：TS254.7[轻工技术与工程—水产品加工及贮藏工程]