生防菌BJ1离子辐照突变高效菌株筛选及对黄瓜枯萎病的生防效果  

Screening of Mutation High-Yielding Biocontrol Bacterium BJ1 by Ion Beam Irradiation and Effect of Controlling Fusarium oxysporum cucunerinum Disease

作　　者：马爽[1,2] 李文建[1] 王菊芳[1] 董喜存[1,2] 余丽霞[1] 刘敬[1] 

机构地区：[1]中国科学院近代物理研究所,甘肃兰州730000 [2]中国科学院研究生院,北京100049

出　　处：《原子能科学技术》2010年第3期376-380,共5页Atomic Energy Science and Technology

基　　金：中国科学院西部之光资助项目(O606180XBO)

摘　　要：枯草芽孢杆菌BJ1是一种在真菌病害防治中发挥重要作用的生防因子,为进一步提高它的抑菌能力,获得生防效果更好的高效菌种,利用不同能量和剂量的^12C^6＋对生防菌BJ1进行了离子辐照处理。研究结果表明：离子辐照生防菌BJ1的最适宜剂量为200-400 Gy,传能线密度（LET）为60 keV/μm;突变菌株的抑菌能力比BJ1提高了2%-21%;不仅防病效果比BJ1提高了17.48%,而且对植物具有更好的促生长作用。BJ1 of Bacillus subtilis is an important biocontrol factor in control of fungus disease.In order to improve the antagonistic ability of the strain,and obtain high-efficiency strains,^2C^6＋ of different doses and linear energy transfer（LET） was used to irradiate the biocontrol bacterium BJ1.The optimum dose and LET of ion beam irradiation for the BJ1 are 200-400 Gy and 60 keV/μm,respectively.The antagonistic ability is increased by 2%-21%.The control effect of mutation to Fusarium oxysporum f.sp.cucunerinum is increased by 17.48% over that of BJ1,and mutation also has better plant growth-promoting effect.

关 键 词：离子辐照 生防菌BJ1 诱变筛选 抑菌防病 

分 类 号：Q691.5[生物学—生物物理学] Q691.8