化学计量学解析法与山柰挥发油化学成分的GC-MS分析  

Analysis of essential oil components in Kaempferia galanga L. by GC-MS and chemometric resolution method

作　　者：佘金明[1,2] 彭友林[3] 梁逸曾[1] 刘汉[2] 华美玲[2] 熊峻[2] 

机构地区：[1]中南大学化学化工学院中药现代化研究中心,湖南长沙410083 [2]常德职业技术学院药学系,湖南常德415000 [3]湖南文理学院生命科学系,湖南常德415000

出　　处：《湖南文理学院学报（自然科学版）》2010年第1期24-27,共4页Journal of Hunan University of Arts and Science(Science and Technology)

摘　　要：分析山柰挥发油中的化学成分;采用水蒸气蒸馏法从山柰中提取挥发油成分,通过气-质联用技术(GC-MS)对其分离检测,利用化学计量学解析法(CRM)分辨重叠色谱峰,同时结合程序升温保留指数辅助定性;共分辨出75个色谱峰,鉴定了其中65个组分,占山柰挥发油总量的97.47%,其中主要化学成分为正十五烷(31.02%),反式桂皮酸乙酯(23.49%)、4-甲氧基桂皮酸乙酯(14.86%)、3-蒈烯(8.83%)等组分,而其它61个组分只占21.56%;结合化学计量学解析法和保留指数分析山柰挥发油化学成分,比单独使用GC-MS其结果更加准确、可靠,有助于山柰的进一步开发利用.The essential oil components in Kaempferia galanga L. is analyze; The volatile constituents in Kaempferia galanga L. were extracted by steam distillation. Gas chromatography-mass spectrometry （GC-MS） combined with Chemometrie Resolution Method（CRM）, a useful chemometric resolution method which can be used to resolve overlapping peaks in chromatogram, was employed to identify and comparatively analyze the volatile constituents, together with the temperature-programmed retention index for assistant identification; A total of 75 chromatographic peaks were resolved, and of these 65 components in the essential oils ofKaempferia galanga L. were identified, which accounted for 97.47% of the total relative contents. The main chemical compositions in the studied sample were Pentadec, anv（31.02%）, （E）-2-Propenoic acid, 3-phenyl-, ethyl ester（23.49%）, （E）-2-Propenoic acid, 3-（4-methoxyphenyl）-, ethyl ester（14.86%）and 3-Carene（8.83%）etc. and the other 61 constituents only accounted for about 21.56% of the total contents; Our work demonstrated that CRM and retention index could provide a complementary and convenient method for accurate analysis of complex essential oils. The results obtained may be helpful for the further exploitation of Kaempferia galanga L..

关 键 词：化学计量学解析法 山柰 挥发油 气相色谱-质谱 

分 类 号：O623.7[理学—有机化学] S662.3[理学—化学]