赤松离体培养植株再生体系的建立  

In vitro plantlet regeneration from seedling explants of Pinus densiflora

作　　者：朱丽华[1,2] 吴小芹[1,2] 戴培培[1,2] 张红岩[1,2] 

机构地区：[1]南京林业大学森林资源与环境学院,江苏南京210037 [2]江苏省有害生物入侵预防与控制重点实验室,江苏南京210037

出　　处：《南京林业大学学报（自然科学版）》2010年第2期11-14,共4页Journal of Nanjing Forestry University：Natural Sciences Edition

基　　金："十一五"国家科技支撑计划(2006BAD08A19122)

摘　　要：以无菌条件下萌发21～28d的赤松(Pinus densiflora)幼苗子叶-胚轴材料为起始外植体建立了植株再生体系。结果表明:在添加4mg/L6-BA和0.05mg/L NAA的GD培养基上培养5周可诱导丛生芽形成,无6-BA但添加0.1mg/L NAA的DCR培养基促进了芽的进一步生长,DCR培养基中添加0.5～1.0g/L活性炭促进了芽的伸长,在添加2mg/L6-BA和0.1～0.2mg/L NAA的GD培养基上丛生芽大量增殖。伸长的丛生芽接种在附加0.2mg/L NAA的1/2GD培养基中培养4周后,不定根发生率达68.4%,转移至无激素但添加0.5g/L活性炭的1/2GD中不定根迅速伸长。将完整的再生植株移栽于蛭石-珍珠岩-河沙等比混合的基质中,12周后成活率约为60%。A plantlet regeneration protocol was developed for P.densiflora by using Cotyledon-hypocotyl explants from 21—28 days old aseptically grown seedlings. The explants were cultured on a GD medium containing 4 mg/L 6-BA （6-benzylaminopurine） and 0.05 mg/L NAA （α-naphthylacetic acid） for 5 weeks to obtain bud induction. Bud growth was enhanced by the elimination of 6-BA and the inclusion of 0.1 mg/L NAA in DCR medium. Shoots elongation was achieved on DCR medium supplemented with 0.5—1.0 g/L activated charcoal （AC）. Multiple shoots in subcultures were proliferated on GD medium with 2 mg/L BAP and 0.2 mg/L NAA. Root primordia were induced in 68.4% of shoots after 4 weeks of culture on 1/2 GD medium containing 0.2 mg/L NAA. Roots elongated rapidly after the transmission of rooted plantlets to 1/2 GD medium with 0.5 g/L AC. The rooted plantlets were successfully transferred to cups containing a mixture of vermiculite-perlite-sand. The surviving rate was approximate 60% after 12 weeks in the greenhouse.

关 键 词：赤松 丛生芽诱导 丛生芽增殖 不定根 植株再生 

分 类 号：S718[农业科学—林学] Q948[生物学—植物学]