低温锻炼对蝴蝶兰组培苗抗寒性的影响  

Effects of Cold Acclimation on Cold Resistance in Plantlets of Phalaenopsis Amabilis

作　　者：马玉涵[1] 崔广荣[2] 赵岩[1] 储俊[1] 周正义[1] 

机构地区：[1]安徽科技学院生命科学学院,安徽凤阳233100 [2]安徽科技学院植物科学学院,安徽凤阳233100

出　　处：《安徽科技学院学报》2010年第1期23-28,共6页Journal of Anhui Science and Technology University

基　　金：安徽科技学院引进人才专项(ZRC2007135);安徽科技学院引进人才专项(ZRC2007147);安徽省自然科学基金项目(070411010);安徽省教育厅省级自然科学基金项目(KJ2007B053)

摘　　要：在模拟冷害条件下,研究了低温锻炼对蝴蝶兰组培苗生理作用的影响,并对低温锻炼的最适温度进行筛选。结果表明,低温胁迫会造成蝴蝶兰组培苗存活率下降,可溶性糖含量、脯氨酸含量、超氧化物歧化酶活力、过氧化氢酶活力上升,丙二醛含量上升。低温锻炼可以提高蝴蝶兰组培苗的存活率,提高蝴蝶兰组培苗渗透性物质含量、抗氧化酶水平、降低丙二醛含量,从而增强了蝴蝶兰组培苗的抗寒能力。其中,17℃/12℃昼夜低温锻炼对模拟冷害具有最为显著的缓解作用。To elucidate the freezing tolerance mechanism of perennial plants,Phalaenopsis amabilis Protocorm-like body was used as model.Activities of antioxidant enzyme and solute contents were investigated in plantlets of Phalaenopsis amabilis.Cold-hardening（17℃/12℃） increased the activities of SOD,CAT in plantlets.A similar increase was also observed in the protein,proline and soluble carbohydrates content.Meanwhile,Cold acclimation decreased the MDA content.These results obtained suggest that the higher activities of SOD,CAT as well as the higher protein,proline,soluble carbohydrates and MDA content may be biochemical adaptation for freezing tolerance in cold-hardened Phalaenopsis amabilis plantlets.In conclusion,17℃/12℃ is the optimal temperature to enhance the cold resistance in Plantlets of Phalaenopsis amabilis.

关 键 词：蝴蝶兰 组培苗 低温锻炼 抗寒性 

分 类 号：S426[农业科学—植物保护]