体外法研究延胡索酸二钠对瘤胃微生物发酵活力及甲烷产量的影响  

Effects of disodium fumarate on in vitro methane production and fermentation of rumen microbial

作　　者：毛胜勇[1] 王新峰[1] 朱伟云[1] 

机构地区：[1]南京农业大学动物科技学院,江苏南京210095

出　　处：《草业学报》2010年第2期69-75,共7页Acta Prataculturae Sinica

基　　金：国家自然科学基金项目(30530560)资助

摘　　要：为探讨延胡索酸二钠对瘤胃甲烷产量及瘤胃微生物发酵活力的影响,本研究共设计了3个试验来阐明该问题。试验1采用体外批次培养,研究了不同日粮条件下(高牧草日粮、中等水平牧草的日粮和低牧草日粮)添加延胡索酸二钠(0,4和7 mmol/L)对山羊瘤胃微生物发酵及甲烷产量的影响。结果表明,较对照相比,添加延胡索酸二钠显著提高了累积产气量、pH值和TVFA产量(P<0.05),降低了甲烷产量(P<0.05),其中高牧草日粮组下降幅度最大。试验2探讨了延胡索酸二钠对黄化瘤胃球菌发酵粗饲料活力的影响。结果表明,与对照组相比;添加延胡索酸二钠显著提高了黄化瘤胃球菌数量及其对黑麦草的降解率(P<0.05);试验3探讨了延胡索酸二钠对瘤胃真菌发酵粗饲料活力的影响,结果显示,延胡索酸二钠显著降低了厌氧真菌发酵的总产气量、干物质消失率及羧甲基纤维素酶酶活(P<0.05)。结果说明,延胡索酸二钠在降低甲烷产量方面与发酵底物的天然特性有关,其中对高牧草日粮的作用效应最为显著;延胡索酸二钠可提高瘤胃混合微生物与瘤胃纤维降解菌发酵粗饲料的能力,但对瘤胃真菌的发酵活力具有抑制效应。Three experiments were conducted to investigate the effects of disodium fumarate on CH4 production and fermentation by rumen micro-organisms.In experiment 1,rumen contents were collected from three local goats.Disodium fumarate was added to the incubation bottles to achieve final concentrations of 0,4 and 8 mmol/L-fumarate.Results showed that disodium fumarate addition increased the final pH and the production of acetate and propionate linearly（P0.001）,and reduced the amount of NH3-N in the cultures.Compared with the control,adding disodium fumarate to batch cultures reduced CH4 production.In experiment 2,the effect of disodium fumarate on the fermentation activity of Ruminococcus flavefaciens was investigated.Disodium fumarate was added to the incubation bottles to achieve final concentrations of 0,4,7 and 10 mmol/L.Disodium fumarate addition increased the dry matter loss,TVFA production,and the counts of R.flavefaciens.In experiment 3,the effect of disodium fumarate on ryegrass fermentation by rumen fungi was investigated.Disodium fumarate addition（0,4,7,and 10 mmol/L） reduced the dry matter loss and the cumulative gas production（P0.01）.These results indicate that the effects of disodium fumarate on rumen fermentation mainly depend on the nature of the incubated substrate,with the high-forage diet showing the greatest response.Disodium fumarate addition increased the fermentation activity of the mixed rumen micro-organisms and of R.flavefaciens,but reduced the fermentation activity of the rumen fungi.

关 键 词：延胡索酸二钠 瘤胃微生物 甲烷 批次培养 

分 类 号：S816[农业科学—饲料科学]