眼镜蛇毒组分C的抗瘤活性及其对肿瘤细胞存活率的影响  

Antitumour Activity of Fraction C of Venom of Naja naja atra and its Effect on BALB/C Tumour Cell Survival Rate

作　　者：植飞 赵路宁 林振桃 管锦霞 

机构地区：[1]广州蛇毒研究所

出　　处：《蛇志》1998年第3期1-3,共3页Journal of Snake

基　　金：广东省科委基金

摘　　要：目的观察眼镜蛇毒组分Ｃ对ＢＡＬＢ／Ｃ型小鼠腹水型肝癌Ｈ２２的抑瘤作用及其体外对肝癌Ｈ２２细胞存活率的直接影响。方法通过半体内实验，观察眼镜蛇毒组分Ｃ对小鼠腹水型肝癌Ｈ２２的抑瘤率；通过细胞培养，以细胞存活率为指标观察中华眼镜蛇毒组分Ｃ对ＢＡＬＢ／Ｃ型小鼠腹水型肝癌Ｈ２２细胞在体外的直接杀伤作用。结果眼镜蛇毒组分Ｃ能明显抑制ＢＡＬＢ／Ｃ型小鼠腹水型肝癌Ｈ２２细胞的生长，其抑瘤作用随剂量增大而增强，ＩＣ５０为９５ｍｇ／Ｌ。在体外，眼镜蛇毒组分Ｃ的浓度对ＢＡＬＢ／Ｃ型小鼠腹水型肝癌Ｈ２２细胞存活率的影响随其作用剂量的增大而增强，ＩＣ５０为９ｍｇ／Ｌ；同时，这种影响还随其作用时间的延长而增强。结论眼镜蛇毒组分Ｃ对ＢＡＬＢ／Ｃ型小鼠腹水型肝癌Ｈ２２有显著的抑瘤作用。Objective To observe the antitumour activity of fraction C of venom of Naja naja atra on BALB/C mouse hepatoma cells Methods By half in vivo experiment,the rates of tumour weight in hibition on mouse hepatoma H 22 were investigated With the method of cells culture,using the cell survival rates as a sign to test the direct effect of fraction C on BALB/C mouse hepatoma H 22 cells in vitro Results Fraction C can obviously inhibit the survival of BALB/C mouse hepatoma H 22 cells and its inhibitive effects on mouse hepatoma H 22 increase when the dosage increases and its IC 50 is 95 mg/L The effects of fraction C on BALB/C mouse Hepatoma H 22 depend on its concentration and action time Conclusions Fraction C has obvious antitumour activity on mouse hepatoma

关 键 词：中华眼镜蛇毒组分C 腹水型肝癌细胞 细胞存活率 

分 类 号：R735.7[医药卫生—肿瘤] R282.740.5[医药卫生—临床医学]