甘氨酸对延边黄牛体细胞克隆重组胚发育的影响  

Effect of glycine on the development of somatic cell cloned reorganization embryo of Yanbian yellow cattle

作　　者：韩岩[1] 魏丽荣[1,2] 孙丽丽[1,2] 张涛[1] 李鹏飞 王洪宝 李钟淑[1] 方南洙[1] 

机构地区：[1]延边大学农学院,吉林龙井133400 [2]首都医科大学附属北京天坛医院药剂科,北京100000 [3]吉林省龙井市畜牧兽医局,吉林龙井133400

出　　处：《中国兽医学报》2010年第3期411-413,共3页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(30860184);延边大学211工程三期立项项目

摘　　要：为了提高延边黄牛体细胞克隆重组胚的发育率,研究了在体外培养液中添加不同浓度的甘氨酸对重组胚发育的影响。分别在体外培养液中添加0、7、14、21mmol/L的甘氨酸,结果表明,在体外培养液中添加甘氨酸能够显著提高延边黄牛体细胞克隆重组胚的发育率,且以7mmol/L浓度的甘氨酸囊胚的发育率最高。In order to improve the efficiency of Yanbian yellow cattle somatic cell cloned recombinant embryos development,The study of the in vitro culture medium added（0,7,14,21 mmol/L） on the impact of in vitro culture medium with different concentrations of glycine the reorganization embryo development.Adding glycine could increase the cleavage rate of embryos reorganization,but 7 mmol/L glycine displayed the highest rate of blastocyst development,decreased in the other groups.In vitro culture medium added glycine can significantly enhance somatic cell cloning reorganization of embryo growth rate Yanbian yellow cattle.

关 键 词：延边黄牛 体细胞克隆 甘氨酸 

分 类 号：S823.31[农业科学—畜牧学]