鼻咽癌组织中EB病毒LMP1与hsa-miR-19b表达及其相关性分析  

Expression and correlation of LMP1 and has-miR-19b in nasopharyngeal carcinoma

作　　者：肖胜军[1,2] 张小玲[2] 韦敏怡[1] 

机构地区：[1]广西医科大学基础学院,南宁530021 [2]桂林医学院基础学院

出　　处：《山东医药》2010年第14期25-27,共3页Shandong Medical Journal

基　　金：广西高校人才小高地病理学创新团队基金资助项目

摘　　要：目的观察鼻咽癌(NPC)组织中EB病毒潜伏膜蛋白1(LMP1)和hsa-miR-19b表达变化及其与NPC临床病理特征的关系。方法采用免疫组化技术检测46例NPC放疗前活检标本中LMP1的表达情况,用实时定量PCR检测hsa-miR-19b表达情况。分析二者表达相关性及其与NPC临床病理特征的关系。结果NPC组织中LMP1阳性表达率为60.95%,hsa-miR-19b阳性表达量为68.27±69.00;LMP1、hsa-miR-19b表达均与NPC分期和淋巴结转移有关。LMP1表达与hsa-miR-19b表达呈正相关(r=0.390,P=0.007)。结论LMP1可能调控hsa-miR-19b的表达,两者的表达呈正相关关系。LMP1、hsa-miR-19b的高表达与NPC分期和转移有关。Objective To study the relationship between the expression of LMP1 and hsa-miR-19b; and the relationship between their expression and clinicopathological parameters. Methods LMP1 of 46 cases of nasopharyngeal carcinoma was detected by immunohistochemisty and hsa-miR-19b by RT-PCR. Results The positive expression rate of LMP1 was 60.95% （28/46）. The relative expression value of hsa-miR-19b was 68.27±69.00. Both LMPI and hsa-miR-19b were associated with stage and lymphatic metastasis. The expression of LMP1 were positively associated with the expression of hsa-miR-19b（r = 0.390, P = 0.007）. Conclusions LMP1 may play a regulation role in the expression of hsa-miR-19b and their expression are possitively correlation. High expression of LMP1 and hsa-miR-19b may have prognosis significance in staging and metastasis in nasopharyngeal carcinoma.

关 键 词：鼻咽癌 EB病毒 潜伏膜蛋白1 has-miR-19b 

分 类 号：R739.63[医药卫生—肿瘤]