siRNA干扰整合素αvβ3表达抑制脑胶质瘤细胞U251增殖的实验研究  

In vitro study on siRNA integrin αvβ3 gene expression inhibiting glioma U251 cells proliferation

作　　者：赵天书[1] 王策[1] 王雪峰[1] 董白晶[1] 孙庆喜 张尧[3] 李利[1] 刘晓谦[1] 

机构地区：[1]哈尔滨医科大学附属第四医院神经外科,150001 [2]黑龙江省绥化市第一人民医院神经外科 [3]北京三博医院神经外科

出　　处：《中华神经外科杂志》2010年第1期86-90,共5页Chinese Journal of Neurosurgery

基　　金：基金项目：黑龙江省科技厅计划项目（GB04C3023）

摘　　要：目的探索特异性siRNA靶向干扰恶性胶质瘤U251细胞中INTαvβ3表达后对细胞生长的作用。方法将INTαvβ3特异性siRNA经脂质体（LipofectamineTM2000）转染U251细胞。RT—PCR、免疫细胞化学和Westernblot法检测INTαvβ3蛋白表达。MTT检测干扰后细胞增殖变化。结果INTαvβ3特异siRNA对U251细胞增殖有明显抑制作用。siRNA组、脂质体组与对照组各时间点的结果差异有统计学意义。免疫细胞化学对照组和脂质体组均见INTαvβ3呈绿色表达，siRNA组表达降低。Westernblot法和RT—PCR结果证实转染特异siRNA后的细胞在蛋白及mRNA水平均能抑制INTαvβ3的表达。结论特异性siRNA可干扰INTαvβ3在脑胶质瘤U251细胞中的表达并抑制细胞增殖。Objective Explore the specificity of siRNA interference targeting malignant glioma U251 cells, INTαvβ3 expression of cell growth after the role. Method U251 cells were transfected with INT αvβ3specific siRNA by lipofectamine（LipofectamineTM2000）. RT- PCR,immune cells staining and Western blot were used to determine the expression of INTαvβ3. Cell proliferation after the detection of changes in interference was tested by MTT. Results INTαvβ3 specific siRNA shown to inhibit U251 cell proliferation. There were statistical significance between siRNA group, liposome group and the control group at different time points. Green INTαvβ3 expression was found in control group and liposome groups, and reduced in siRNA group. Western, blotting and RT - PCR results shown that the expression of integrin could be inhibited by αvβ3transfection in the protein and mRNA level. Conclusions Integrin - specific siRNA INTαvβ3can inhibit the U251 glioma cell proliferation.

关 键 词：SIRNA 整合素ΑVΒ3 神经胶质瘤 U251细胞 

分 类 号：R739.4[医药卫生—肿瘤]