不同浓度氧合血红蛋白对平滑肌细胞核因子-κB DNA结合活性的影响  

Effect of oxyhemoglobin on the DNA - binding activity of nuclear factor-κB in cultured vascular smooth muscle cells

作　　者：印红霞[1] 周梦良[1] 杭春华[1] 祁晓平[1] 史继新[1] 

机构地区：[1]南方医科大学南京临床学院(南京军区南京总医院)神经外科,210002

出　　处：《中华神经外科杂志》2010年第2期116-119,共4页Chinese Journal of Neurosurgery

基　　金：江苏省自然科学基金资助（BK2007153）

摘　　要：目的观察氧合血红蛋白（OxyHb）对培养的血管平滑肌细胞（VSMCs）细胞密度及核因子（NF）-κB的DNA结合活性的影响。方法用不同浓度（1～100μmol／L）的OxyHb处理VSMCs，观察其形态学及细胞密度变化，测定NF-κB的DNA结合活性。结果OxyHb刺激后VSMCs都表现出形态学的改变，其严重程度有浓度依赖性。但是在24h时只有10（3μmol／L组有明显的细胞数量减少，10μmol／L组在48h时细胞数量开始明显减少。NF-κB的DNA结合活性在经OxyHb处理后明显增高，以10μmol／L及50μmol／L组最高。结论NF-κB的DNA结合活性在经OxyHb处理的VSMCs中有明显改变，揭示了NF-κB在脑血管痉挛发生时是脑血管平滑肌细胞内重要的信号通路。Objective To investigate the effect of oxyhemoglobin （OxyHb） on the cell density and DNA - binding activity of nuclear factor （NF） -κB in the cultured vascular smooth muscle cells（VSMCs）. Methods The cultured VSMCs were treated with different concentration from 1 to 100 μmol/L of OxyHb. The cell density was measured. DNA - binding activity of NF -κB was detected using electrophoretic mobility shift assay （EMSA）. Results Morphological alterations occurred in the VSMCs after treatment with OxyHb. The concentration of OxyHb at 100 μmol/L produced a significant decrease in cell density at 24 hour, and 10 μmol/L at 72 hour. DNA - binding activity of NF -κB was increased after treatment with OxyHb, and peaked at 10 and 50 μmol/L. Conclusion Alteration of NF -κB DNA - binding activity occurred in the VSMCs treating with OxyHb, which suggests that NF -κB pathway in the cerebrovascular smooth muscle cells may be involved in the pathogenesis of cerebral vasospasm.

关 键 词：脑血管痉挛 蛛网膜下腔出血 氧合血红蛋白 平滑肌细胞 核因子-ΚB 

分 类 号：R743.3[医药卫生—神经病学与精神病学]