射频消融治疗大鼠移植性肝肿瘤对脾脏树突状细胞的影响  

The influence of radiofrequency ablation of liver tumors on spleen dendritic cells

作　　者：戴维德[1] 范智慧[2] 陈敏华[2] 河福金[3] 李洪民[3] 

机构地区：[1]卫生部北京医院超声科,北京100730 [2]北京大学临床肿瘤学院超声科 [3]北京大学临床肿瘤学院病理科

出　　处：《中华物理医学与康复杂志》2010年第2期106-109,共4页Chinese Journal of Physical Medicine and Rehabilitation

基　　金：北京市科委重大项目培育专项（Z0005190040431）

摘　　要：目的探讨射频消融（RFA）治疗大鼠肝肿瘤对其脾脏组织内树突状细胞（DC）表型的影响及其意义。方法选取40只正常Sprague Dawley大鼠，10只作为正常对照组，其余30只制作Walker-256肝肿瘤模型后，随机分为RFA后7d组、RFA后14d组和荷瘤对照组，每组10只。RFA后7d组、RFA后14d组分别于RFA处理后7d及14d处死，荷瘤对照组与正常对照组不做RFA处理即处死。取其脾脏组织，采用Ficoll密度梯度离心法分离出单个核细胞，应用流式细胞术检测单个核细胞OX62、OX6及CD86表达水平。结果正常大鼠与荷瘤大鼠的脾脏单个核细胞中分别有（10．36±3．21）％、（11．69±4．39）％表达OX62，二者比较差异无统计学意义（P〉0．05）。正常大鼠与荷瘤大鼠的脾脏单个核细胞中分别有（76．33±7．86）％、（60．58±6．26）％表达OX6；而分别有（63．06±8．77）％、（40．87±8．66）％表达CD86，2者比较差异均有统计学意义（P〈0．05）。RFA后7dOX62阳性表达率为（10．24±2．49）％，RFA后14d为（15．10±2．37）％，RFA后14d组与荷瘤对照组比较差异有统计学意义（P〈0．05）。RFA后7dOX6阳性表达率为（77．45±4．53）％，RFA后14d为（75．47±5．22）％，2组与荷瘤对照组比较差异均有统计学意义（P〈0．05）。RFA后7dCD86阳性表达率为（46．86±9．42）％，RFA后14d为（45．53±9．13）％，2组与荷瘤对照组比较差异均无统计学意义（P〉0．05）。结论荷瘤鼠脾脏组织中未成熟DC比例高，抗原提呈能力弱，RFA促使荷瘤大鼠外周血中DC前体细胞迁移到脾脏组织中的数量增多，并进一步分化成熟，对提高机体在免疫应答中的抗原提呈能力起到促进作用。Objective To study the influence of radio-frequency ablation （RFA） of liver tumors on the phenotype of spleen dendritic cells in rats. Methods Ten healthy Sprague Dawley rats constituted the normal control group. Thirty experimental Walker-256 liver tumor model rats were prepared by implantating tumor particles, and these were then randomly divided equally into three groups： a 7 days after RFA group, a 14 d after RFA group and a tumor control group. The spleen tissue of all groups was sampled before the experiment, and the spleens of the 7 d and 14 d after RFA groups were sampled again 7 d and 14 d after RFA respectively. The mono- nuclear cells in the spleen tissue were separated through Ficoll density gradient centrifugation. The expression level of OX62, OX6 and CD86 in the mononuclear spleen cells was analyzed with flow cytometry. Results In the spleens of the normal control rats （ 10.36 ±3.21 ） % of mononuclear cells expressed OX-62. In the tumor control group the proportion was （ 11.69 ± 4.39） % , and the difference between the two groups was not significant. In the spleens of the normal rats （76.33 ± 7.86）% of mononuclear cells expressed OX-6, while in the tumor control group the ratio was （60. 58 ± 6.26）%, a significant difference. In the spleens of the normal rats （63. 06 ± 8.77） % of mononuclear cells expressed CD-86, while in the tumor control rats the ratio was （40.87±8.66）% , a difference which was not significant. In spleens of rats with tumors 7 d and 14 d after RFA the proportion of OX- 62 was （ 10.24 ± 2.49 ） % and （ 15. 10± 2.37） % respectively. The difference between the tumor control group and the 14 d after RFA group was significant. The proportion of OX-6 in the 7 d and 14 d groups was （77.45 ± 4.53） % and （75.47 ± 5.22） % respectively, and both levels were significantly different from the tumor control group. The proportion of CD86 was （46.86±9.42）% and （45.53 ±9. 13）% in the 7 d and 14 d RFA groups respectively, and neither

关 键 词：射频消融 大鼠 肝肿瘤 脾脏 树突状细胞 

分 类 号：R735.7[医药卫生—肿瘤]