低分子肝素对种植性乳腺癌生长和转移的抑制作用  

Inhibitory effect of low molecular weight heparin on the growth and metastasis of human mammary carcinoma cells implanted in nude mice

作　　者：刘飞[1] 李汉军[1] 李介秋[1] 汤恢焕[3] 刘庚勋[2] 葛健君[1] 孔屏[1] 

机构地区：[1]解放军第163医院普通外科,长沙410003 [2]解放军第163医院病理科,长沙410003 [3]中南大学湘稚医院普通外科

出　　处：《中华实验外科杂志》2010年第1期50-52,共3页Chinese Journal of Experimental Surgery

摘　　要：目的 观察低分子肝素在种植性乳腺癌动物模型中对肿瘤生长和转移的抑制作用.方法 制作120例MCF-7乳腺癌细胞移植瘤裸鼠模型,将其随机分为低分子肝素组、生理盐水组、化疗组、内分泌治疗组,每组30只;观察裸鼠一般情况及移植瘤的牛长变化,28 d后统一脱颈椎处死裸鼠,切取移植瘤标本进行常规镜检及应用免疫组织化学检测瘤组织内血管内皮生长因子（VEGF）和微血管密度（MVD）的变化.结果 实验4周后,低分子肝素组、化疗组、内分泌治疗组不仅在移植瘤体积、胸壁浸润及淋巴转移等方面明显优于生理盐水组,其VEGF、MVD的表达亦低于生理盐水组,差异有统计学意义（P〈0.01）;其中尤以低分子肝素组中VEGF、MVD的表达更低,但与化疗组、内分泌治疗组间对比差异并无统计学意义（P〉0.01）.结论 低分子肝素在裸鼠MCF-7移植瘤模型中可以通过抑制肿瘤血管形成而抑制移植瘤的生长及转移.Objective To study the inhibitory effect of low molecular weight heparin （LMWH） on the growth and metastasis of breast cancer （BC） cells in the MCF-7 nude mice models.Methods BC models were successfully established in 120 nude mice by implanting human MCF-7 cells,and randomly divided into 4 groups：chemotherapy group,LMWH group,endocrine group and normal saline （NS） group.During 4 weeks of treatment,the general physical state of the nude mice and the development of the tumors were observed.After 28 days,the model mice were sacrificed by taking off the cervical vertebra,and the pathological changes of the samples were observed and the expression of vascular endothelial growth factor （VEGF）and microvessel density（MVD）in the tumor samples was detected by immunohistochemicalmethod respectively.Results After 4 weeks of treatment,the expression levels of vascular endothelial growth factor （VEGF） and MVD in chemotherapy group,LMWH group and endocrine group were significantly lower than those in NS group（P〈0.01）,and they were superior to NS group in the aspects of tumor volume,chest wall erosion and lymph node metastasis.Among all these groups,the expression levels of VEGF and MVD in LMWH group were decreased markedly,but there Was no statistically significant difference.Conclusion LMWH Can inhibit the growth and metastasis of BC in the MCF-7 nude mice models.

关 键 词：乳腺癌 低分子肝素 血管内皮生长因子 血管生成 

分 类 号：R737.9[医药卫生—肿瘤]