直肠癌组织中miR-155和miR-200c的表达差异及意义  

The differential expression of miR-155 and miR-200c in rectal cancer tissues and significance

作　　者：张鹏[1] 周总光[1] 王玲[1] 王默进[1] 张益[1] 崔长富[1] 詹兰[1] 

机构地区：[1]四川大学华西医院生物治疗国家重点实验室消化外科研究室,成都610041

出　　处：《中华实验外科杂志》2010年第1期59-61,共3页Chinese Journal of Experimental Surgery

基　　金：美国中华医学会基金资助项目（CMB96636）

摘　　要：目的 分析人直肠癌组织miR-155和miR-200c的表达差异及其与直肠癌临床病理特征的关系.方法 选取手术切除的卣肠癌标本71例,以正常黏膜为对照,采用实时荧光定量-聚合酶链反应（qRT-PCR）对miR-155和miR-200c作定量分析.结果 相对于正常黏膜组织,miR-155和miR-200c在直肠癌组织中表达上调,差异有统计学意义（P〈0.05）.miR-155的表达与患者年龄有关（P〈0.05）;miR-200c的表达与患者血清癌胚抗原（CEA）水平有关（P〈0.05）.不同的直肠癌性别、临床分期、浸润深度、淋巴结转移、远处转移和分化程度间miR-155、miR-200c表达的差异无统计学意义（P〉0.05）.结论 miR-155和miR-200c在直肠癌组织中显著表达,可能参与直肠癌的发生过程.miR-200c对于直肠癌町能具有潜在的辅助诊断、疗效判断及预后评估意义.Objective To elucidate the miR-155 and miR-200c expression in rectal callcers,and analyze the correlation of their expression with clinicopathological features.Methods Real-time PCR was used to quantify the miR-155 and miR-200c expression in 71 pairs of rectal cancer and normal mucosa samples,and the relationship of their expression and clinicopathological features was analyzed.Results The miR-155 and miR-200c expression in rectal cancers was up-regulated compared to normal mucosa （P〈0.05 respectively）.Furthermore.the miR-155 expression was related to the age of rectal cancer patients（P〈0.05）,and the miR-200c expression was related to serum Careino-embryonic antigen （CEA） level（P〈0.05）.There was no relationship between miR-155/miR-2130c and gender,TNM staging,local invasion,lymph metastasis,distant metastasis and cell differentiation（P〉0.05）.Condusion The miR-155 and miR-200c overexpression may play acertain roleinthe development of rectal cancer.MiR-200c may represent a potential indicator in the auxiliary diagnosis,therapeutic effect and prognosis of rectal cancer.

关 键 词：直肠癌 实时荧光定量聚合酶链反应 MICRORNA 

分 类 号：R735.37[医药卫生—肿瘤]